摘要
目的探讨三氧自血回输联合腰交感神经双极射频治疗慢性盆腔痛效果及其对患者炎症因子及TGF-β/Smads通路蛋白的影响。方法前瞻性连续选取2020年7月至2022年7月阜阳市妇女儿童医院收治的慢性盆腔痛患者82例,随机数字表法分为A组(30μg/mL浓度医用三氧自血回输+85℃、60 s/次腰交感神经双极射频热凝治疗,n=41)和B组(30μg/mL浓度医用三氧自血回输治疗,n=41)。治疗前、治疗后1周、4周时均采用视觉模拟评分(VAS)进行疼痛情况评价。治疗前、治疗后4周时取空腹静脉血并采用酶联免疫吸附法检测血清TNF-α、IL-1β、CRP、IL-6等炎症因子水平及血清TGF-β/Smads通路蛋白Smad3、Smad7、TGF-β1水平。观察比较两组不良反应发生率。结果A组治疗1周和4周的VAS评分均低于B组,差异有统计学意义(t=5.486,2.531,P=<0.001,0.013),且两组治疗1周和4周的VAS评分均低于同组治疗前,差异有统计学意义(t=12.027,23.655,6.355,19.097,P<0.001)。治疗后4周B组与A组的炎症因子及TGF-β/Smads通路蛋白比较差异均有统计学意义(t=7.063,3.471,4.688,8.832,11.749,9.986,9.401,均P<0.01),且两组组内治疗前、治疗后4周的血清TNF-α、IL-1β、CRP、IL-6、TGF-β1、Smad3和Smad7水平比较差异均有统计学意义(t=17.617,13.469,20.012,27.010,20.229,18.390,28.829,9.246,10.339,21.048,16.345,15.784,16.860,24.404,P<0.01)。两组不良反应发生率比较差异无统计学意义(χ^(2)=0.499,P=0.480)。结论30μg/mL浓度医用三氧自血回输联合85℃、60 s/次腰交感神经双极射频热凝治疗慢性盆腔痛安全可靠,而炎症因子及TGF-β/Smads通路蛋白调节可能为其作用机制。
Objective To investigate the effect of medical ozone autologous blood transfusion combined with lumbar sympathetic nerve bipolar radiofrequency treatment in patients with chronic pelvic pain,and its influence on inflammatory factors and TGFβ/Smads pathway proteins.Methods 82 patients with chronic pelvic pain admitted from July 2020 to July 2022 were prospectively selected and randomly divided in⁃to group A(30μg/mL concentration of medical ozone autologous blood transfusion+85℃,60 s/time bipolar radiofrequency thermocoagulation of lumbar sympathetic ganglia,n=41)and group B(30μg/mL concentra⁃tion of medical ozone autologous blood transfusion,n=41).Visual analogue scale(VAS)was used to evalu⁃ate the pain before treatment and 1 day,1 week,2 weeks and 4 weeks after treatment.Fasting venous blood was taken at the same time and serum inflammatory factors such as TNF⁃α,IL⁃1β,CRP and IL⁃6 levels were detected by enzyme⁃linked immunosorbent assay,and Smads related protein and TGF⁃β1 positive expression rate in venous blood centrifugal precipitate was detected by immunohistochemistry method.The incidence of adverse reactions between the two groups was observed and compared.Results The VAS scores of 1 and 4 weeks after treatment in group A were significantly lower than those in Group B(t=5.486,2.531,P<0.001).Moreover,the VAS scores of the two groups at 1 and 4 weeks of treatment were lower than those of the same group before treatment,with statistically significant differences(t=12.027,23.655,6.355,19.097,P<0.001).Inflammatory factors and TGFβ/Smads pathway proteins at 4 weeks after treatment in Group B and Group A were statistically significant(t=7.063,3.471,4.688,8.832,11.749,9.986,9.401,P<0.01).And before treatment and at 4 weeks after treatment,the serum levels of TNF⁃α,IL⁃1β,CRP,IL⁃6,TGF⁃β1,Smad3 and Smad3 in the same group of the two groups were statistically significant different(t=17.617,13.469,20.012,27.010,20.229,18.390,28.829,9.246,10.339,21.048,16.345,15.784,16.860,24.404,P<0.
作者
王莉
王家松
汝占魁
WANG Li;WANG Jiasong;RU Zhankui(Department of Pain,Fuyang Women and Childrens Hospital,Fuyang,Anhui,China,236000)
出处
《分子诊断与治疗杂志》
2023年第5期842-845,共4页
Journal of Molecular Diagnostics and Therapy
基金
阜阳市科技局项目(FK20208120)。
关键词
三氧自血回输
腰交感神经射频
慢性盆腔痛
炎症因子
TGF⁃β/Smads通路
Medical ozone autologous blood transfusion
Radiofrequency of lumbar sympathetic nerve
Chronic pelvic pain
Inflammatory factors
TGF⁃β/Smads pathway
