摘要
目的探讨羟基红花黄色素A(hydroxysafflor yellow A,HSYA)对慢性束缚应激模型小鼠抑郁样行为及海马γ-氨基丁酸(γ-aminobutyric acid,GABA)A型受体(type A GABA receptor,GABAAR)表达的影响。方法将健康雄性SPF级C57BL/6C小鼠按照随机数字表法分为对照组、HSYA组、模型组、模型+HSYA组、模型+氟西汀组,每组12只。采用慢性束缚应激方法建立抑郁小鼠模型,HSYA组和模型+HSYA组小鼠腹腔注射给予HSYA(20 mg/kg),模型+氟西汀组小鼠腹腔注射氟西汀(10 mg/kg),对照组和模型组小鼠给予等体积0.9%氯化钠溶液,1次/d,连续给药14 d。采用强迫游泳实验和悬尾实验评价动物的抑郁样行为,Western blot检测小鼠海马组织内GABAAR不同亚型的蛋白表达水平。采用SPSS 19.0和GraphPad Prism 8.0软件分析数据和制图,多组间比较采用单因素方差分析,进一步两两比较采用Tukey-HSD检验。结果(1)在行为学实验中,5组小鼠在强迫游泳实验中的漂浮不动时间和悬尾实验中的悬尾不动时间均差异有统计学意义(F=21.59,20.81,均P<0.05)。模型组小鼠漂浮不动时间[(143.91±9.97)s]和悬尾不动时间[(107.00±6.54)s]均高于对照组[(52.92±6.70)s,(43.50±5.96)s,均P<0.05]。模型+HSYA组小鼠的游泳漂浮不动时间[(26.17±7.69)s]和悬尾不动时间[(20.17±7.89)s]与模型+氟西汀组[(61.60±16.22)s,(34.14±10.74)s]相比均差异无统计学意义(均P>0.05),但两组的游泳漂浮时间和悬尾不动时间均低于模型组(均P<0.05)。(2)Western blot结果显示,4组小鼠海马组织内GABAAR亚型GABAARβ1和GABAARβ2的蛋白表达水平均差异有统计学意义(F=12.21,11.40,均P<0.05)。模型组小鼠海马组织内GABAARβ1(45.60±10.76)和GABAARβ2(46.27±4.82)的蛋白表达水平均低于对照组[GABAARβ1(100.00±3.44),GABAARβ2(100.00±3.26),均P<0.05]。与模型组相比,模型+HSYA组GABAARβ1(79.91±5.00)和GABAARβ2(79.08±5.53)蛋白表达水平均较高(均P<0.05)。此外,4组小鼠海马组织�
Objective To investigate the effects of hydroxysafflor yellow A(HSYA)on depressive-like behavior and expression of type Aγ-aminobutyric acid receptor(GABAAR)in hippocampus of chronic restraint stress model mice.Methods The SPF grade male C57BL/6C mice were divided into Control group,HSYA group,Model group,Model+HSYA group and Model+fluoxetine group according to random number table method,with 12 mice in each group.Mice model of depression was established by chronic restraint stress.Mice in HSYA group and Model+HSYA group were intraperitoneally injected with HSYA(20 mg/kg),mice in Model+fluoxetine group were injected intraperitoneally with fluoxetine(10 mg/kg),and mice in Control group and Model group administered with 0.9%sodium chloride solution intraperitoneally once a day for 14 days.Then,the forced swimming test(FST)and tail suspension test(TST)were performed to evaluate the depressive-like behavior of mice,and the protein expression levels of different subtypes of GABAAR in the hippocampus of mice were determined by Western blot.SPSS 19.0 and GraphPad Prism 8.0 software were used for data statistical analysis and mapping.One-way ANOVA was used for comparison among groups,and Tukey-HSD test was used for further pairwise comparison.Results(1)In the behavioral tests,there were significant differences in swimming immobility time of FST and tail suspension immobility time of TST among the five groups(F=21.59,20.81,both P<0.05).The swimming immobility time((143.91±9.97)s)and tail suspension immobility time((107.00±6.54)s)in Model group were higher than those in Control group((52.92±6.70)s,(43.50±5.96)s,both P<0.05).There were no significant difference in swimming immobility time and tail suspension immobility time between Model+HSYA group((26.17±7.69)s,(20.17±7.89)s)and Model+fluoxetine group((61.60±16.22)s,(34.14±10.74)s)(both P>0.05),but the swimming immobility time and tail suspension immobility time in these two groups were lower than those in Model group(both P<0.05).(2)The Western blot results showe
作者
李红
侯灵芝
李松阳
张佳丽
李媛媛
吴启今
李海锦
张悦
王佳卉
成军
王芳
李彩
Li Hong;Hou Lingzhi;Li Songyang;Zhang Jiali;Li Yuanyuan;Wu Qijin;Li Haijin;Zhang Yue;Wang Jiahui;Cheng Jun;Wang Fang;Li Cai(Department of Psychiatry,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Pharmacy,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2023年第5期393-398,共6页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金(81801335,81801325)
河南省中青年卫生健康科技创新杰出青年人才培养项目(YXKC2020035)。
