摘要
为进一步发掘野生大豆中的优质抗逆基因以用于大豆的种质改良,本研究选取积极参与调控植物生长和非生物胁迫抗性的扩展蛋白基因为对象,在野生大豆中克隆了GsEXPA3基因并对其进行了生物信息学分析,通过qRT-PCR检测该基因在逆境胁迫下的转录情况,采用大豆毛状根转化试验分析其对于根系生长的调控作用。结果显示:启动子分析表明GsEXPA3基因的转录可能参与光、脱落酸及干旱胁迫响应并与类黄酮物质合成相关。在低温和干旱胁迫下,GsEXPA3基因的转录呈显著增加趋势,分别在处理后12和9 h达到最高点,为对照组的3.27倍和2.09倍。GsEXPA3基因的过表达显著促进了转基因大豆毛状根的生长,毛状根数量、总根长和总根重,与对照组相比分别提高了56.83%、53.19%和53.35%。结果说明野生大豆扩展蛋白基因GsEXPA3对于栽培大豆的分子育种具有良好的应用价值。
To further explore the superior stress resistance genes in wild soybean for germplasm improvement in soybean,the expansin genes that are actively involved in regulating plant growth and abiotic stress resistance were selected for this study.This study cloned the GsEXPA3 gene from wild soybean,and performed bioinformatics analysis.We detected the transcription of this gene under stress by qRT-PCR,and carried out the hairy root transformation test of soybean to analyze its regulatory effect on root growth.The results were as follows,promoter analysis revealed that transcription of GsEXPA3 gene may be involved in light,abscisic acid and drought stress responses and correlated with flavonoid synthesis.The transcription of GsEXPA3 gene showed a significant increasing trend under low temperature and drought stress,reaching its highest levels at 12 and 9 h after treatment,respectively,which were 3.27 and 2.09 fold of the control.Overexpression of GsEXPA3 significantly enhanced hairy root growth in transgenic soybeans,with the number of hairy roots,total root length,and total root weight increased by 5683%,53.19%,and 53.35%,respectively,compared with the control.The results indicate that wild soybean expansin gene GsEXPA3 has good application value for molecular breeding of cultivated soybean.
作者
李翠婷
王琳琳
李凤兰
贺付蒙
王雪
李丽
王丽娟
冯旭
LI Cui-ting;WANG Lin-lin;LI Feng-lan;HE Fu-meng;WANG Xue;LI Li;WANG Li-juan;FENG Xu(College of Life Sciences,Northeast Agricultural University,Harbin 150030,China;College of Agriculture,Northeast Agricultural University,Harbin 150030,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2023年第3期268-275,共8页
Soybean Science
基金
国家自然科学基金青年基金(32201717)
国家大豆产业技术体系岗位科学家(CARS-04-PS21)
中国博士后科学基金(2022MD713729)
黑龙江省博士后基金(LBH-Z21008)
东北农业大学青年才俊人才项目(54982912)。
