摘要
为了解不同放牧强度下天山北坡中段伊犁绢蒿(Seriphidiumtransiliense)荒漠草地土壤细菌群落的组成和结构特征,本研究通过植物群落调查和土壤微生物高通量测序技术对轻度放牧(LG)和重度放牧(HG)处理下荒漠草地生态系统土壤细菌群落组成及土壤理化性质进行研究,探讨放牧对草地生态系统土壤细菌群落和土壤理化因子的影响。结果表明:(1)从土壤细菌多样性指数来看,HG显著降低了土壤细菌群落的Chao1和ACE物种丰富度指数(P<0.05),但对Shannon多样性指数和Simpson多样性指数无显著影响(P>0.05)。(2)从土壤细菌群落结构来看,LG显著提高了放线菌门(Actinobacteria)和绿弯菌门(Chloroflex)的相对丰度,HG显著提高了变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和厚壁菌门(Firmicutes)的相对丰度(P<0.05)。(3)在HG和LG水平下土壤理化性质并未出现显著变化;(4)从土壤细菌群落与土壤理化性质的冗余分析发现,土壤全氮是导致土壤细菌群落发生变化的决定性因素。该结果揭示了放牧条件下伊犁绢蒿荒漠草地土壤细菌群落的变化,同时为荒漠草地土壤与土壤细菌群落结构及多样性之间的作用机制提供理论依据,为荒漠草地生态系统的利用、保护与修复提供参考。
The objective of the study was to elucidate the effect of the composition and structure of the soil bacterial community in the desert grassland on Seriphidium transiliense in the middle part of the northern slope of the Tianshan Mountains under different grazing intensities.In this study,we investigated the composition of the soil bacterial community and soil physical and chemical properties of desert grassland ecosystems under light grazing(LG)and heavy grazing(HG)treatments using plant community surveys and soil microbial high-throughput sequencing technology.We explored the effects of grazing on soil bacterial communities and soil physical and chemical factors in grassland ecosystems.The results indicated:(1)From the perspective of the soil bacterial diversity index,HG decreased the Chao1 species richness index and ACE species richness index(P<0.05),but had no substantial effect on the Shannon diversity index and Simpson diversity index(P>0.05).(2)From the soil bacterial community structure perspective,LG increased the relative abundance of Actinobacteria and Chloroflex,and HG increased the relative abundance of Proteobacteria,Acidobacteria,and Firmicutes(P<0.05).(3)Soil physical and chemical properties did not change substantially at HG and LG levels.4)From the analysis of the relationship between the soil bacterial community and soil physical and chemical properties,it was evident that soil total nitrogen was the decisive factor leading to changes in the soil bacterial community.Our results reveal the changes in the soil bacterial community in the desert grassland of Seriphidium transiliense under grazing conditions,provide a theoretical basis for the mechanism of interaction between the soil and the soil bacterial community structure and diversity in the desert grassland,and provide a reference for the utilization,protection,and restoration of desert grassland ecosystems.
作者
江康威
张青青
王亚菲
李宏
杨永强
吐尔逊娜依·热依木
JIANG Kangwei;ZHANG Qingqing;WANG Yafei;LI Hong;YANG Yongqiang;Tursunnayi·Reyimu(Grass Industry College,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;College of Life Sciences,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;College of Resource and Environment,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China)
出处
《草业科学》
CAS
CSCD
北大核心
2023年第5期1243-1257,共15页
Pratacultural Science
基金
国家自然科学基金(31960338)
东北师范大学植被生态科学教育部重点实验室开放项目(130026533)。
关键词
放牧
天山北坡
伊犁绢蒿荒漠草地
土壤细菌群落
土壤理化性质
冗余分析
驱动因素
grazing
northern slope of Tianshan Mountains
desert grassland of Seriphidium transiliense
soil bacterial community
soil physical and chemical properties
redundancy analysis
driving factors
