摘要
目的 收集中日友好医院106例诺卡菌病患者的108株非重复性诺卡菌,并分析其菌种分布及抗菌药物敏感耐药情况。方法 通过基质辅助激光解吸电离-飞行时间质谱或16S rRNA靶向测序鉴定诺卡菌菌属,采用微量肉汤稀释法测定诺卡菌对15种抗菌药物的敏感耐药情况,总结2例同时感染2种诺卡菌的肺诺卡菌病患者和3例肺移植后肺诺卡菌病患者的临床特征。结果 在108株诺卡菌菌株中,从痰液和支气管肺泡灌洗液分别分离出诺卡菌67株(62.0%)、32株(29.6%)。皮疽诺卡菌(35.2%)、盖尔森基兴诺卡菌(32.4%)和脓肿诺卡菌(11.1%)是分离数居前3位的诺卡菌菌种。所有诺卡菌菌株均未对利奈唑胺耐药,仅有2.6%的皮疽诺卡菌对甲氧苄啶-磺胺甲噁唑(TMP-SMZ)耐药。结论 诺卡菌菌株对TMP-SMZ和利奈唑胺均高度敏感,但不同诺卡菌菌种对抗菌药物的耐药性特征不同。
Objective To collect 108 non-repetitive Nocardia strains from 106 patients with nocardiosis in China-Japan Friendship Hospital,and to analyze the distribution and antimicrobial susceptibility of the strains.Methods Nocardia species was identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry or 16S rRNA targeted sequencing.Broth microdilution method was used to determine the susceptibility and resistance of Nocardia to 15 antibiotics.The clinical features of 2 cases of pulmonary nocardiosis co-infected with 2 Nocardia species and 3 cases of pulmonary nocardiosis after lung transplantation were summarized.Results Among the 108 Nocardia strains,67(62.0%)and 32(29.6%)Nocardia strains were isolated from sputum and bronchoalveolar lavage fluid,respectively.Nocardia farcinica(35.2%),Nocardia gelsenkirchen(32.4%)and Nocardia abscessus(11.1%)were the top three Nocardia strains.None of the Nocardia strains were resistant to linezolid.Only 2.6%of the Nocardia strains were resistant to trimethoprim-sulfamethoxazole(TMP-SMZ).Conclusion Nocardia strains are highly sensitive to TMP-SMZ and linezolid,but different Nocardia species have different resistance characteristics to antibiotics.
作者
胡晏宁
王利君
鲁炳怀
HU Yanning;WANG Lijun;LU Binghuai(Peking University China-Japan Friendship School of Clinical Medicine,Beijing 100029,China;Laboratory of Clinical Microbiology and Infectious Diseases,China-Japan Friendship Hospital,Beijing 100029,China;Department of Clinical Laboratory,Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghua University,Beijing 102218,China)
出处
《国际检验医学杂志》
CAS
2023年第12期1435-1441,共7页
International Journal of Laboratory Medicine
基金
首都卫生发展科研专项基金(2020-2-4062)。
关键词
下呼吸道感染
诺卡菌
诺卡菌病
肺移植
甲氧苄啶-磺胺甲噁唑
lower respiratory tract infection
Nocardia
nocardiosis
lung transplantation
trimethoprim-sulfamethoxazole
