摘要
为明确云南玉溪烟区烟草黑胫病菌生理小种的类别及对主栽品种的致病性,以来源于玉溪市新平县和澄江市的172株烟草疫霉菌为材料,采用TTC法及活体植株接种法明确其生理小种的归属和组群分布,以不同生理小种的烟草疫霉菌株为接种体,研究生理小种鉴定接种技术并验证其可靠性。结果表明,TTC平板上呈现红色的供试烟草疫霉菌株均属于0号或1号生理小种;活体植株接种显示,0号和1号生理小种有149株和12株,分别占比86.63%和6.98%;无致病力及未鉴定出小种归属的菌株分别有2株和9株,分别占比1.16%和5.23%。表明新平县和澄江市烟草黑胫病菌以0号生理小种为主。致病性测定显示,170个菌株对主栽品种KRK26、云烟87和K326均有较强致病力。此外,寄主离体叶片与茎秆轻微致伤后,对烟草黑胫病菌生理小种的抗感反应与活体植株注射接种结果一致,具有同等的鉴别力。说明离体材料致伤接种的方法可用于区分不同烟草品种对黑胫病的抗感性。
To investigate the physiological race classification and pathogenicity regarding main cultivars of Phytophthora parasitica var.nicotianae in Yuxi tobacco-growing areas of Yunnan,172 strains of P.parasitica var.nicotianae were isolated from Xinping County and Chengjiang City in Yuxi.Their physiological races and group distribution were identified with TTC method and in vivo plant inoculation method.Using P.parasitica var.nicotianae strains of different physiological races as inoculums,the inoculation technology for physiological race identification was studied,and its reliability was verified.The results showed that the tested strains were magenta on TTC plate and belonged to physiological race 0 or 1.In vivo plant inoculation results indicated that there were 149 strains of race 0,12 strains of race 1,2 non-pathogenic strains,and 9 unidentified strains,accounting for 86.63%,6.98%,1.16%,and 5.23%,respectively,which suggested that race 0 was the main physiological race of P.parasitica var.nicotianae in Xinping County and Chengjiang City.The pathogenicity test results showed that 170 strains had strong pathogenicity to the main cultivars KRK26,Yunyan 87 and K326.In addition,the resistant or susceptible reaction of slightly injured in vitro leaves and stalks of the host to the physiological race of P.parasitica var.nicotianae was consistent with that of in vivo plants after inoculation injection,and they had equivalent discriminability.In conclusion,the wound inoculation method of in vitro materials can be used to distinguish the resistance of different tobacco varieties to black shank disease.
作者
刘迎龙
王军伟
陆俊平
何鹏搏
何鹏飞
卢灿华
李鹏强
吴毅歆
孔宝华
田阳阳
何月秋
LIU Yinglong;WANG Junwei;LU Junping;HE Pengbo;HE Pengfei;LU Canhua;LI Pengqiang;WU Yixin;KONG Baohua;TIAN Yangyang;HE Yueqiu(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming 650201,China;Hongta Tobacco(Group)Co.,Ltd.,Yuxi 653100,Yunnan,China;Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650021,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2023年第6期17-23,共7页
Tobacco Science & Technology
基金
红塔烟草(集团)有限责任公司资助项目“玉溪典型烟区黑胫病发生风险评估及其应用研究”(HTJT-2020-0487)、“特殊香气风格烟叶开发及其应用研究”(2020-YL06)。
关键词
烟草黑胫病菌
生理小种
鉴定
致病性
离体接种
Phytophthora parasitica var.nicotianae
Physiological race
Identification
Pathogenicity
In vitro inoculation
