摘要
农药广泛且长期持续性用于农业生产中,而农药利用率仅有40%左右,60%进入水土环境中,造成农药污染问题。溴氰虫酰胺作为广泛使用的双酰胺类杀虫剂,对土壤非靶标生物具有潜在风险。为研究溴氰虫酰胺对土壤环境生物的影响,本研究选取赤子爱胜蚓为模式生物,通过转录组学的方法,研究0.1、10、100 mg/kg干土溴氰虫酰胺对赤子爱胜蚓的生态毒性。研究发现,随着溴氰虫酰胺的浓度增加,其差异基因数目增多;GO富集分析和KEGG富集分析发现,富集的通路也在增加,溴氰虫酰胺对蚯蚓的转录水平影响增大。在GO富集分析的三大分支中,溴氰虫酰胺主要影响了55个二级功能条目;在KEGG富集分析中,溴氰虫酰胺主要影响6条一级代谢通路。高浓度处理100 mg/kg干土的溴氰虫酰胺主要引起蚯蚓的抗氧化应激反应,进而影响蚯蚓的糖类和脂质的合成和代谢相关基因表达,最终影响消化系统、循环系统和内分泌系统的相关基因的表达。本研究通过转录组学了解了溴氰虫酰胺对蚯蚓的分子毒理学,为溴氰虫酰胺毒理学研究提供参考。
Pesticides are widely used in agricultural production,and the pollution of pesticides are becoming more and more serious for a long time,but the utilization rate of pesticide is only about 40%,60%pesticides into the soil and water environment,resulting in the problem of pesticide pollution gradually serious.Cyantraniliprole is a widely used diamide insecticide with potential risk to soil non-target organisms.In order to study the effects of cyantraniliprole on soil environment organisms,we selected the earthworm(Eisenia fetida)as the model organism,and studied the ecotoxicity of cyantraniliprole with different concentrations to earthworm by transcriptomics method.It was found that the number of differentially expressed genes increased with the increase of the concentration of cyantraniliprole,and the enriched pathways also increased through GO enrichment analysis and KEGG enrichment analysis,indicating that the effect of cyantraniliprole on the transcription level of earthworm increased.The results showed cyantraniliprole mainly affected 55 secondary functional items in the three branches of GO enrichment analysis.In KEGG enrichment analysis,cyantraniliprole mainly affected 6 primary metabolic pathways.In general,the high concentration 100 mg/kg soil of cyantraniliprole treatment mainly caused the antioxidant stress reaction of earthworms,affected the expression of related genes in the synthesis and metabolism of sugars and lipids,and finally affected the expression of related genes in the digestive system,circulatory system and endocrine system in earthworms.In this study,the molecular toxicology of cyantraniliprole to earthworms was studied through transcriptome,which provided a reference for the toxicology research of cyantraniliprole.
作者
乔治华
刘翔
姚向峰
杜庆志
张建业
孙石昂
张风文
姜兴印
QIAO Zhihua;LIU Xiang;YAO Xiangfeng;DU Qingzhi;ZHANG Jianye;SUN Shiang;ZHANG Fengwen;JIANG Xingyin(College of Plant Protection,Shandong Agricultural University,Tai’an271018,China;Key Laboratory of Pesticide Toxicology&Application Technique,Tai'an271018,Shandong,China;Tobacco)
出处
《现代农药》
CAS
2023年第3期78-86,共9页
MODERN AGROCHEMICALS
基金
山东省现代农业产业技术体系(SDAIT-02-10)
山东“双一流”奖补项目(SYL2017-XTTD11)。
关键词
溴氰虫酰胺
转录组
蚯蚓
环境
GO富集分析
KEGG富集分析
cyantraniliprole
transcriptome
earthworm
environment
GO enrichment analysis
KEGG enrichment analysis
