摘要
该研究对8种中国传统干腌肉制品[四川风干牛肉肽(BP)、内蒙古风干羊肉肽(MP)、金华火腿肽(HP)、江西咸鸭肽(DP)、湖北风干鸡肉肽(CP)、安徽风干鹅肽(GP)、鲢鱼风干肽(SCP)和风干黄鱼肽(YCP)]进行了抗氧化和抗癌活性的评价。用DPPH自由基、ABTS阳离子自由基和·OH检测其抗氧化能力。结果表明,CP、SCP和YCP具有较好的抗氧化活性,CP(1 mg/mL)对DPPH自由基的清除率为(87.1±1.7)%,YCP(1 mg/mL)对ABTS阳离子自由基的清除率为(49.6±0.5)%,SCP(1 mg/mL)对·OH的清除率为(48.3±1.0)%。DP、CP和BP对肿瘤细胞有较强的抑制作用,BP(4 mg/mL)对SH-SY5Y细胞的抑制率达(48.45±0.81)%。用BP验证BP对SH-SY5Y细胞周期的影响及其对细胞凋亡的影响,认为BP中存在使SH-SY5Y细胞阻滞于S期的特异性多肽可能是引起细胞凋亡的主要原因。所以,中国传统干腌肉制品可能在抗氧化以及抗癌方面促进人体健康。
In this study,eight traditional Chinese dried and cured meat products[Sichuan air-dried beef peptide(BP),Inner Mongolia air-dried lamb peptide(MP),Jinhua ham peptide(HP),Jiangxi salted duck peptide(DP),Hubei air-dried chicken peptide(CP),Anhui air-dried goose peptide(GP),silver carp air-dried peptide(SCP),and air-dried yellow croaker peptide(YCP)]were evaluated for their antioxidant and anticancer activities.The antioxidant capacity was measured by DPPH radicals,ABTS cationic radicals,and·OH.Results showed that CP,SCP,and YCP had good antioxidant activities,and the scavenging rate of DPPH radicals by CP(1 mg/mL)was(87.1±1.7)%,the scavenging rate of ABTS cationic radicals by YCP(1 mg/mL)was(49.6±0.5)%,and the scavenging rate of hydroxyl radicals by SCP(1 mg/mL)was(48.3±1.0)%.DP,CP,and BP had strong inhibitory effects on tumor cells,and the inhibition rate of BP(4 mg/mL)on SH-SY5Y cells reached(48.45±0.81)%.The effect of BP on the SH-SY5Y cell cycle and its effect on apoptosis was verified with BP,and it was concluded that the presence of specific peptides in BP that blocked SH-SY5Y cells in the S phase might be the main cause of apoptosis.Therefore,Chinese traditional dry-cured meat products may promote human health in terms of antioxidants as well as anti-cancer.
作者
张迎阳
陈文涛
邹平
郑文武
ZHANG Yingyang;CHEN Wentao;ZOU Ping;ZHENG Wenwu(College of Food,Changzhou University,Changzhou 213164,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第11期183-190,共8页
Food and Fermentation Industries
基金
常州市科技攻关计划项目(CE20212030,CE20212039)。
关键词
干腌肉
生物活性肽
自由基
抗癌
抗氧化
dry cured meat
bioactive peptides
free radicals
anticancer
antioxidation
