摘要
【目的】多数桃品种果实在成熟后迅速软化,导致来不及采收、采后易腐烂,是制约桃产业发展的关键问题。慢溶质桃具有较长的硬熟期,可以减轻因果实软化造成的损耗,是未来桃耐贮运育种的重要方向之一。【方法】以慢溶质品种春雪、春瑞为试材,通过构建群体、肉质评价,对慢溶质性状进行遗传分析和集群分离分析(bulk segregant analysis,BSA)定位。【结果】通过对比慢溶质品种和非慢溶质品种成熟过程中软化的特点,发现慢溶质留树时间较长,乙烯延迟释放。在慢溶质分离群体中,留树时间呈明显的双峰分布,遗传倾向分析表明慢溶质受到单基因或主效数量性状基因座(quantitative trait locus,QTL)控制,春雪慢溶质位点为杂合,春瑞为纯合。以春雪桃自交群体为试验材料,基于BSA分析,将慢溶质定位到桃第4号染色体4个区段,总长度7.87 Mb。【结论】桃慢溶质性状遗传受显性单基因或主效基因控制,其控制基因位于第4号染色体。
【Objective】Peach is a specific seasonal supply fruit,its maturity period of fruits is concentrated in just a few days,and fruit softening goes fast in late development,affecting harvest,transportation and shelf life.Slow-melting flesh(SMF)peaches can be kept on the trees for a longer time with high firmness during the ripening period,and reduce the loss due to the fruit softening.The study aimed to understand the inheritance of the SMF character in peach.【Methods】The slow-melting flesh peach varieties Chunli and Chunrui,non-slow-melting flesh(NSMF)peach varieties Chunmei and Chunmi were used as test materials,and grown in Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences.All the fruits of the the 4 varieties were collected at normal maturity.The fruits were covered with foamed nets and shipped back to the laboratory in boxes.The samples were taken every 3 days,and 20 fruits with the same size and uniform coloring were selected for each time.The samples were fixed to determine the ethylene release rate,fruit firmness.According to the physiological changes of fruits of the SMF and NSMF,the softening characteristics of the SMF peaches were clarified.The genetic population was constructed with the slow-melting flesh varieties Chunxue and Chunrui.The fruit hardness of the offsprings was investigated every 2 days from May to August of 2021 and 2022 in the Xinxiang Experimental Base of Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences.Days on the tree after fruit ripening were analyzed,and≥10 days were considered as a physiological index for determining SMF fruits.Furthermore,the inheritance was analyzed and the gene was mapped by Bulked Segregant Analysis(BSA).From Chunxue selfing segregation population,20 plants with for NSMF trait and 20 plants with SMF trait were selected.The DNA was extracted,and two DNA mixing pools(dominant pool:SMF and recessive pool:NSMF)were constructed separately.The constructed two DNA mixing pools were used for sequencing.The Raw
作者
孟君仁
牛良
潘磊
崔国朝
孙世航
段文宜
曾文芳
王志强
MENG Junren;NIU Liang;PAN Lei;CUI Guochao;SUN Shihang;DUAN Wenyi;ZENG Wenfang;WANG Zhiqiang(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences/National Peach and Grape Improvement Center/Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs,Zhengzhou 450009,Henan,China)
出处
《果树学报》
CAS
CSCD
北大核心
2023年第6期1064-1071,共8页
Journal of Fruit Science
基金
河南省重大公益科技专项(201300110500)
中国农业科学院郑州果树研究所科研业务费专项(ZGS202209)
河南省优秀青年科学基金项目(212300410094)
中国农业科学院科技创新工程(CAAS-ASTIP-2023-ZFRI)
河南省科技攻关计划项目(202102110037)。
关键词
桃
果实
慢溶质
软化
Peach
Fruit
Slow-melting flesh
Softening
