摘要
目的:探讨绒毛膜促性腺激素对复发性流产大鼠的治疗效果,为临床中优化复发性流产的治疗方案提供理论基础。方法:选取未交配生育的SPF级雌性大鼠共40只,随机分为对照组(A组)、模型组(B组)、常规治疗组(C组)、绒毛膜促性腺激素治疗组(D组),每组各10只。雌鼠妊娠后开始干预。A组大鼠全天仅灌服生理盐水。B组大鼠上午灌服生理盐水,C组大鼠上午灌服地屈孕酮水溶液,D组大鼠上午注射绒毛膜促性腺激素注射液,下午B、C、D组大鼠灌服羟基脲溶液。妊娠第十天给予各组大鼠灌服米非司酮。比较各组大鼠胚胎吸收率、炎性因子水平、免疫因子水平、雌激素受体表达水平及子宫内膜损伤程度。结果:干预后,与A组相比,各组大鼠胚胎吸收率增高,与B组大鼠相比,C组、D组大鼠的胚胎吸收率降低,且D组大鼠低于C组;与A组相比,各组大鼠IL-17水平、IL-17/TGF-β、Th17细胞比例、Th17/Treg升高,且TGF-β水平、Treg细胞比例下降,同时C组、D组大鼠的IL-17水平、IL-17/TGF-β、Th17细胞比例、Th17/Treg均低于B组,且D组低于C组,同时TGF-β水平、Treg细胞比例高于B组,且D组高于C组;干预后,与A组相比,各组大鼠子宫内膜厚度下降,纤维化面积比例增加,与B组相比,C组、D组大鼠的子宫内膜厚度增加且D组高于C组,同时子宫内膜纤维化比例低于B组,且D组低于C组;与A组相比,各组大鼠ER、PR、PRLR mRNA表达水平明显降低,与B组相比,C组、D组大鼠的ER、PR、PRLR mRNA表达水平有所增加,且D组高于C组,差异均具有统计学意义(P<0.05)。结论:绒毛膜促性腺激素可能通过调节炎性因子水平和免疫微环境,提高性激素、受体表达,进而修复子宫内膜损伤,降低胚胎吸收率,促进复发性流产的恢复。
Objective:To investigate the therapeutic effect of chorionic gonadotropin on recurrent abortion in rats,and to provide a theoretical basis for optimizing the treatment of recurrent abortion in clinical practice.Methods:Forty unmated SPF female rats were randomly divided into control group(group A),model group(group B),conventional treatment group(group C)and chorionic gonadotropin treatment group(group D),with 10 rats in each group.The intervention began after the female was pregnant.Rats in group A were only given normal saline throughout the day.Rats in group B were given normal saline in the morning,rats in group C were given dydrogesterone in the morning,rats in group D were given chorionic gonadotropin injection in the morning,and rats in groups B,C,and D were given hydroxyurea solution in the afternoon.On the tenth day of gestation,rats in each group were given mifepristone.The embryonic absorption rate,inflammatory factor level,immune factor level,estrogen receptor expression level and endometrial injury degree of each group were compared.Results:After the intervention,the embryo absorption rate was increased in each group compared with group A,and lower in group C and D rats compared with group B rats,and group D rats were lower than group C rats;Compared with group A,the levels of IL-17,IL-17/TGF-β,the proportion of Th17 cells and Th17/Treg in each group were increased,and the levels of TGF-β and the proportion of Treg cells were decreased.At the same time,the levels of IL-17,IL-17/TGF-β,Th17 cell ratio and Th17/Treg in groups C and D were lower than those in group B,and the levels of TGF-β and Treg cell ratio in group D were higher than those in group B,and those in group D were higher than those in group C.After intervention,compared with group A,the endometrial thickness of rats in each group decreased and the proportion of fibrosis area increased.Compared with group B,the endometrial thickness of rats in groups C and D increased,and the proportion of endometrial fibrosis in group D was higher tha
作者
刘晓庆
刘振国
石晓琳
刘英英
刘亚倩
刘雨婷
LIU Xiao-qing;LIU Zhen-guo;SHI Xiao-lin;LIU Ying-ying;LIU Ya-qian;LIU Yu-ting(Department of Pharmacy,Northwest Women's and Children's Hospital,Xi'an,Shaanxi,710061,China)
出处
《现代生物医学进展》
CAS
2023年第10期1843-1848,共6页
Progress in Modern Biomedicine
基金
2020年陕西省社会科学基金年度项目(2020M005)。
