摘要
以内蒙古地区兴安落叶松分布的3个林区及华北落叶松分布的4个林区为采样点。以兴安落叶松和华北落叶松的根系为研究对象,采用组织分离法从根段分离培养得到根系内生真菌,结合形态特征和rDNAITS区序列分析进行综合鉴定。然后对不同树种及同一树种不同样地的根系内生真菌群落组成和多样性进行差异分析。结果显示,共分离得到225株可培养内生真菌,分别隶属于3纲4目9科17属31种,其中,深色有隔内生真菌(dark septate endophytes,DSE)类群的分离占比最大,是主要的内生真菌类群。两种落叶松根系共有的内生真菌类群有4种,分别为Cadophora echinata、Phialocephala fortinii、Phi.helvetica和Pleotrichocladium opacum。其中Phi.fortinii和Phi.helvetica分别为兴安落叶松和华北落叶松根系内生真菌的优势种。同时,两种落叶松的根系特有内生真菌物种各不相同,群落结构相似程度较低。华北落叶松根系内生真菌多样性指数和均匀度指数均大于兴安落叶松。兴安落叶松分布的3个样地真菌群落均匀度较低。同一树种不同样地间的相似性系数表明样地地理位置及林分条件相近时群落组成相似程度高。综上所述,两种落叶松根系内生真菌组成存在一定的差异,同一树种不同样地的真菌群落结构也具有明显的不同,但整体内生真菌资源都非常丰富。
Three forest regions of Larix gmelinii and four forest regions of L.gmelinii var.principis-rupprechtii in Inner Mongolia were selected as sampling points.Endophytic fungi in root segments of the two larch species were isolated and cultured by tissue isolation methods,and identification of the fungi was based on morphological characteristics and rDNA ITS region sequence analysis.The community composition and diversity of root endophytic fungi in different tree species and different plots of the same tree species were analyzed.The results were that 225 isolates of culturable endophytic fungi were obtained,belonging to 31 species of 17 genera in 9 families of 4 orders and 3 classes.Among them,dark septate endophytes(DSE)accounted for the largest proportion and were the main endophytic fungal group.Cadophora echinata,Phialocephala fortinii,Phi.helvetica and Pleotrichocladium opacum were in common in the roots of the two larch species.Among them,Phi.fortinii and Phi.helvetica were dominant in the roots of L.gmelinii and L.gmelinii var.principis-rupprechtii respectively.The root-specific endophytic fungal species in the two larch species were different,and the similarity of community structure was low.The diversity index and evenness index of endophytic fungi in L.gmelinii var.principis-rupprechtii were higher than those in L.gmelinii.The evenness of fungal community in the three sample plots of L.gmelinii forest region was low.The similarity coefficient between different sites of the same tree species indicated that the community composition was comparatively similar when the geographical location and stand conditions were similar.In conclusion,there were some differences in the composition of endophytic fungi in the roots of the two larch species,and the fungal community structure of the same tree species in different plots were obviously different,but overall,endophytic fungal resources in roots of larches in Inner Mongolia were very rich.
作者
李聪
乌有汗
姚庆智
LI Cong;WU Youhan;YAO Qingzhi(College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2023年第5期1045-1062,共18页
Mycosystema
基金
内蒙古自治区科技计划(2019GG002)
内蒙古农业大学生命科学学院创新团队项目(TD202103)。
关键词
兴安落叶松
华北落叶松
可培养内生真菌
多样性指数
相似性系数
Larix gmelinii
Larix gmelinii var.principis-rupprechtii
culturable endophytic fungi
diversity index
similarity coefficient
