摘要
试验旨在通过体外三步法(瘤胃液法-胃蛋白酶-胰酶)研究绒山羊常用粗饲料原料的体外营养物质降解率。采用单因素完全随机试验设计,比较研究6种粗饲料(玉米秸秆、谷草、苜蓿、玉米青贮、燕麦草、羊草)的营养物质体外降解率。粗饲料原料均在65℃干燥并粉碎过40目筛,在体外模拟瘤胃内环境培养48 h,模拟胃内环境消化1 h,小肠内环境消化24 h,测定粗饲料的干物质(DM)、有机质(OM)、粗蛋白(CP)、粗脂肪(EE)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、半纤维素(HCL)、粗灰分(Ash)和总能(GE),每个处理设6个重复。结果显示,苜蓿和玉米青贮的DM、OM、CP、EE瘤胃-胃-小肠体外降解率分别在96、94%、97%、88%以上,DE高于16 MJ/kg,体外瘤胃的NDF、ADF、HCL降解率较高。苜蓿、玉米青贮的DM、OM、CP和EE在绒山羊体外瘤胃的降解贡献率多在70%以上,玉米秸秆最低,低于65%;谷草的OM和EE降解贡献率低于60%。研究表明,苜蓿和玉米青贮的营养价值高于谷草、燕麦草和羊草,玉米秸秆最低。
The experiment was to study the in vitro nutrient degradation rate of commonly used roughage materials for Cashmere goat through a three-step method(rumen fluid method-pepsin-trypsin).A single factor fully randomized experimental design was used to compare in vitro degradation rates of nutrients in six types of roughage(corn straw,cereal grass,alfalfa,corn silage,oat grass and leymus chinensis).The raw materials of roughage were dried at 65℃and crushed before passing through 40 mesh sieve.They were cultured in vitro in simulated rumen environment for 48 h,digested in simulated stomach environment for 1 h,digested in small intestine environment for 24 h.The DM,OM,CP,EE,NDF,ADF,HCL,Ash and GE of roughage were determined,each treatment has six replicates.The results showed that the in vitro degradation rates of DM,OM,CP and EE in rumen-stomach small-intestine of alfalfa and corn silage were 96%,94%,97%and 88%,respectively.DE was higher than 16 MJ/kg,and the degradation rates of NDF,ADF and HCL in the rumen were higher.The degradation contribution rates of DM,OM,CP and EE from alfalfa and corn silage in rumen of Cashmere goats in vitro were mostly over 70%,while corn straw was the lowest,below 65%.The contribution rate of OM and EE degradation of cereal grass was less than 60%.The study indicates that the nutritional value of alfalfa and corn silage is higher than that of cereal grass,oat grass,and sheep grass,while corn straw is the lowest.
作者
赵浩翔
杜霞
闫素梅
赵艳丽
郭晓宇
郭咏梅
ZHAO Hao-xiang;DU Xia;YAN Su-mei;ZHAO Yan-li;GUO Xiao-yu;GUO Yong-mei
出处
《饲料研究》
CAS
北大核心
2023年第9期5-9,共5页
Feed Research
基金
农业农村部公益行业专项课题(项目编号:201003061)。
关键词
绒山羊
体外发酵特性
营养物质降解率
Cashmere goat
in vitro fermentation characteristics
nutrient degradation rate
