摘要
近年来,神经细胞直接重编程技术已经成为潜在的细胞替代治疗新手段。体内直接重编程利用与缺失细胞相邻的内源细胞再生目标细胞进行替换,可避免由外部细胞移植引起的免疫排斥反应,有效降低致癌风险,具有良好的应用前景。但同时,神经细胞直接重编程也面临着诸多挑战,如重编程后的神经元存活困难、需要损伤或疾病期间丢失的特定神经元亚型的再生、重编程效率低下等。如何实现高效的在体神经细胞直接重编程,是目前需要克服的难题。该综述主要对如何提高神经细胞直接重编程的效率进行概述,主要包括抗氧化剂、铁死亡抑制剂和抗凋亡蛋白的应用、激活神经元富含的线粒体基因、运用表观遗传因子和转录因子组合等,这些将为更好地发挥神经细胞直接重编程的效用提供参考。
In recent years,direct neuronal reprogramming technology has become a potential new choice for cell replacement therapy.Direct reprogramming in vivo converts endogenous cells into desired target cells,avoiding the immune rejections caused by exogenous cell transplantation and effectively reducing the risk of developing cancers.At the meanwhile,however,direct neuronal reprogramming in vivo is faced with much challenge such as low neuronal survival rate,low reprogramming efficiency,and the necessity to regenerate specific neuronal subtypes lost during injury or diseases.This review mainly summarizes the progress on improving the efficiency of direct neuronal reprogramming by means of the applications of the antioxidants,ferroptosis inhibitors,anti-apoptotic proteins,the activation of neuron-enriched mitochondrial genes,and the use of epigenetic factors and combination of transcription factors,which will be of immense help for its future usage.
作者
杜淑娴
莫李存
程乐平
DU Shu-Xian;MO Li-Cun;CHENG Le-Ping(Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry,Guangxi Key Laboratory of Regenerative Medicine,Guangxi Medical University,Nanning 530021,China;Institute of Neuroscience and Guangxi Key Laboratory of Brain Science,Guangxi Health Commission Key Laboratory of Basic Research on Brain Function and Disease,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China;Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education,Nanning 530021,China)
出处
《生命科学》
CSCD
北大核心
2023年第4期419-428,共10页
Chinese Bulletin of Life Sciences
基金
广西科技基地和人才专项(AD21075052)
广西研究生教育创新项目(YCSW20211122)。
关键词
细胞分化
直接重编程
细胞代谢
线粒体
染色质
转录因子
cellular differentiation
direct reprogramming
cellular metabolism
mitochondrion
chromatin
transcription factor
