摘要
目的分析脑胶质瘤组织中微小RNA⁃4744(miR⁃4744)、右开放阅读框激酶2(RIOK2)表达情况及对患者预后的预测价值。方法纳入2014年12月—2017年11月河北北方学院附属第一医院神经外科收治脑胶质瘤患者102例,术中收集脑胶质瘤组织及相对应的癌旁正常组织,实时荧光定量PCR法检测miR⁃4744、RIOK2信使RNA(mRNA)表达水平,免疫组化检测RIOK2蛋白表达。分析脑胶质瘤组织miR⁃4744、RIOK2 mRNA表达水平与患者临床病理特征的关系,miR⁃4744与RIOK2 mRNA表达水平的相关性,miR⁃4744、RIOK2 mRNA表达水平与预后的关系,脑胶质瘤患者预后的影响因素,miR⁃4744、RIOK2 mRNA表达水平对患者术后5年内死亡的预测价值。结果与癌旁组织比较,脑胶质瘤组织中miR⁃4744表达水平明显降低,RIOK2 mRNA和蛋白阳性率均明显升高[t(χ^(2))P=44.282/<0.001、15.538/<0.001、101.868/<0.001];脑胶质瘤组织miR⁃4744、RIOK2 mRNA表达水平与肿瘤分级、远处转移、卡氏功能状态(KPS)评分有关(miR⁃4744:χ^(2)/P=33.443/<0.001、45.773/<0.001、37.920/<0.001,RIOK2:χ^(2)/P=11.453/0.001、16.650/<0.001、16.592/<0.001);脑胶质瘤组织miR⁃4744与RIOK2 mRNA表达水平呈负相关(r/P=-0.636/<0.001);miR⁃4744低表达组患者5年生存率低于miR⁃4744高表达组,RIOK2 mRNA高表达组患者5年生存率低于RIOK2 mRNA低表达组(χ^(2)/P=20.874/<0.001、10.437/0.001);肿瘤分级Ⅲ~Ⅳ级、有远处转移、KPS评分<80分、miR⁃4744低表达、RIOK2 mRNA高表达是脑胶质瘤患者5年内死亡的独立危险因素[HR(95%CI)=2.224(1.297~3.813)、2.442(1.408~4.237)、2.815(1.506~5.261)、2.140(1.173~3.907)、2.114(1.129~3.958)];与存活组比较,死亡组脑胶质瘤组织miR⁃4744表达水平明显降低,RIOK2 mRNA表达水平明显升高(t/P=8.146/<0.001、3.324/0.001);miR⁃4744、RIOK2 mRNA、两者联合预测患者5年内死亡的曲线下面积(AUC)分别为0.752、0.711、0.885,两者联合预测患者5年内死亡的AUC高于单
Objective To analyze the expression of microRNA⁃4744(miR⁃4744)and right open reading frame ki⁃nase 2(RIOK2)in glioma tissue and their predictive value for patient prognosis.Methods From December 2014 to Novem⁃ber 2017,102 patients with glioma were admitted to the Neurosurgery Department of the First Affiliated Hospital of Hebei North University.During the operation,glioma tissue and corresponding normal tissue adjacent to cancer were collected.The expression levels of miR⁃4744 and RIOK2 messenger RNA(mRNA)were detected by real⁃time fluorescent quantitative PCR,and RIOK2 protein expression was detected by immunohistochemistry.Analyze the relationship between the expression lev⁃els of miR⁃4744 and RIOK2 mRNA in glioma tissue and the clinical pathological characteristics of patients,the correlation be⁃tween miR⁃4744 and RIOK2 mRNA expression levels,the relationship between miR⁃4744 and RIOK2 mRNA expression levels and prognosis,the influencing factors of prognosis in glioma patients,and the predictive value of miR⁃4744 and RIOK2 mRNA expression levels on patient mortality within 5 years after surgery.Results Compared with adjacent tissues,the ex⁃pression level of miR⁃4744 in glioma tissue was significantly reduced,and the positive rates of RIOK2 mRNA and protein were significantly increased(t/χ^(2)/P=44.282/<0.001,15.538/<0.001,101.868/<0.001).The expression levels of miR⁃4744 and RIOK2 mRNA in glioblastoma tissue were correlated with tumor grade,distant metastasis,and Kappa's functional state(KPS)score(miR⁃4744:t/P=33.443/<0.001,45.773/<0.001,37.920/<0.001;RIOK2:11.453/0.001,16.650/<0.001,16.592/<0.001).There is a negative correlation between miR⁃4744 and RIOK2 mRNA expression level in glioma tissue(r/P=-0.636/<0.001).The 5⁃year survival rate of patients in the miR⁃4744 low expression group is lower than that in the miR⁃4744 high expression group,and the 5⁃year survival rate of patients in the RIOK2 mRNA high expression group is lower than that in the RIOK2 mRNA low expres
作者
段升强
乔建新
徐新文
刘熙鹏
张秀峰
Duan Shengqiang;Qiao Jianxin;Xu Xinwen;Liu Xipeng;Zhang Xiufeng(Department of Neurosurgery,First Hospital Affiliated to Hebei North University,Hebei Province,Zhangjiakou 075000,China)
出处
《疑难病杂志》
CAS
2023年第6期613-619,共7页
Chinese Journal of Difficult and Complicated Cases
基金
河北省医学科学研究课题计划项目(20190872,20210199)。
