摘要
心脏芯片能够在体外重现心脏组织的动态微环境和生理相似性,有望取代传统的细胞和动物模型,为心脏病理学研究和药物开发等应用提供更可靠的测试平台.目前该领域的研究方向包括改善其微生理可视化特性、增强其仿生性能以及拓展其生物医学应用等.受孔雀羽毛绚丽色彩的启发,本研究制备了一种明亮的MXene结构色水凝胶用于心肌细胞培养,并由此构建了具有光电双响应特性的仿生器官芯片用于激素测试.MXene不仅能够增强结构色的对比度,而且能够赋予该复合水凝胶体系良好的导电性,从而促进其表面培养的心肌细胞的协同搏动.在心肌细胞的驱动下,MXene结构色水凝肢会发生同步的形变,并产生相应的光子带隙和结构色的变化用于反馈心肌细胞的力学行为.研究结果表明了双响应器官芯片在激素毒性测试中的实用价值,为测试模型的开发提供了新的思路.
Heart-on-chips have emerged as a powerful tool to promote the paradigm innovation in cardiac patho-logical research and drug development.Attempts are focused on improving microphysiological visuals,enhancing bionic characteristics,as well as expanding their biomedical applications.Herein,inspired by the bright feathers of peacock,we present a novel optical and electrical dual-responsive heart-on-a-chip based on cardiomyocytes hybrid bright MXene structural color hydrogels for hormone toxicity eval-uation.Such hydrogels with inverse opal nanostructure are generated by using pregel to replicate MXene-decorated colloidal photonic crystal(PhC)array templates.The attendant MXene in the hydrogels could not only enhance the saturation of structural color,but also ensure the composite hydrogel with excellent electroconductivity to facilitate the synergetic beating of their surface cultured cardiomyocytes.In this case,the hydrogels would undergo a synchronous deformation and generate shift in corresponding photonic band gap and structural color,which could be employed as visual signal for self-reporting of the cardiomyocyte mechanics.Based on these features,we demonstrated the practical value of the optical and electrical dual-responsive structural color MXene hydrogels constructed heart-on-a-chip in hormone toxicity testing.These results indicated that the proposed heart-on-a-chip might find broad prospects in drug screening,biological research,and so on.
作者
孙灵钰
汪雨
卞非卡
许冬雨
赵远锦
Lingyu Sun;Yu Wang;Feika Bian;Dongyu Xu;Yuanjin Zhao(Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China;Chemistry and Biomedicine Innovation Center,Nanjing University,Nanjing 210023,China;Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325001,China)
基金
supported by the National Key Research and Development Program of China (2020YFA0908200)
the National Natural Science Foundation of China (T2225003,61927805,and 52073060)
the Nanjing Medical Science and Technique Development Foundation (ZKX21019)
the Clinical Trials from Nanjing Drum Tower Hospital (2022-LCYJ-ZD-01)
Guangdong Basic and Applied Basic Research Foundation (2021B1515120054)
the Shenzhen Fundamental Research Program (JCYJ20190813152616459 and JCYJ20210324133214038)
Jiangsu Funding Program for Excellent Postdoctoral Talent (2022ZB710)
China Postdoctoral Science Foundation (2022TQ0145 and 2022M721581)。
