摘要
本文建立了超高效液相色谱串联三重四极杆质谱法(UPLC-MS/MS)检测药用铝箔类中34种芳香伯胺的含量.采用Waters ACQUITY UPLC-HSS T3(100 mm×2.1 mm I.D.,1.8μm)色谱柱,以0.1%甲酸水溶液-乙腈为流动相,梯度洗脱,流速为0.4 mL·min^(−1),柱温为40℃.采用电喷雾离子源(ESI+),工作模式为多反应监测模式(MRM).34种芳香伯胺在1—100 ng·mL^(−1)浓度范围内均具有较好的线性关系(r>0.999),各化合物保留时间和峰面积的相对标准偏差均小于0.21%和5.00%,加标回收率在73.5%-114.8%之间.该方法具有灵敏度高、重复性好、准确度高的特点,可用于药用铝箔中34种芳香伯胺的测定.
An ultra performance liquid chromatography tandem triple quadrupole mass spectrometry(UPLC-MS/MS)method was developed for the determination of 34 primary aromatic amines in medicinal aluminum foils.Waters ACQUITY UPLC-HSS T3(100 mm×2.1 mm I.D.,1.8μm)chromatographic column was used,with 0.1%formic acid aqueous solution-acetonitrile as mobile phase and gradient elution.The flow rate was 0.4 mL·min^(−1),column temperature was 40℃.Electrospray ion source(ESI+)was used,and the working mode was multi-reaction monitoring mode(MRM).The 34 primary aromatic amines had satisfactory linear relationships in the concentration range of 1—100 ng·mL^(−1)(r>0.999).The relative standard deviations of retention time and peak area of each compound were less than 1.21%and 5.00%,respectively.The recoveries were between 73.5%and 114.8%.The established method has the characteristics of high sensitivity,good repeatability and high accuracy,and is feasible for the determination of 34 primary aromatic amines in medicinal aluminum foil.
作者
邹佳佳
杨乾展
代磊
李舒雅
何丹
ZOU Jiajia;YANG Qianzhan;DAI Lei;LI Shuya;HE Dan(College of Pharmacy,Chongqing Medical University,Chongqing,400016,China;Shimadzu Co.,LTD,Analytical&Measuring Instruments Division,Chongqing,400010,China)
出处
《环境化学》
CAS
CSCD
北大核心
2023年第5期1763-1766,共4页
Environmental Chemistry
基金
重庆医科大学药学院支部书记“双带头人”项目(YXY2021SDTR01)资助.
