摘要
甲状腺乳头状癌(PTC)的发病率正在迅速增加,需要寻找新的生物标志物用于治疗和预后.运动蛋白家族在肿瘤发生和发展中发挥重要作用,是多种肿瘤的治疗靶点.基于GEPIA数据库初步分析KIF23对PTC预后的影响.与正常组织相比,显示KIF23基因在甲状腺癌组织中的表达水平上调.从The Cancer Genome Atlas (TCGA)中下载两组PTC队列,采用Kaplan-Meier方法进行生存分析,发现KIF23高表达组的预后比低表达组的要差.采用免疫组织化学方法检测67例甲状腺癌组织中KIF23的表达及临床病理特征,进一步证实KIF23在甲状腺癌细胞中的表达上调.随后,在甲状腺癌细胞中敲低KIF23,并通过RT-qPCR和western blot检测KIF23在甲状腺癌细胞中的表达证实敲低成功.通过集落形成实验与CCK-8检测,分析KIF23对PTC细胞增殖的影响,结果发现,KIF23敲低表达后甲状腺癌细胞的增殖受到显著抑制.结果提示,KIF23的表达与甲状腺癌细胞的临床病理增殖有关.抑制KIF23基因可抑制甲状腺癌细胞的病理增殖,可能成为未来治疗靶点.KIF23的高表达可作为PTC患者预后不良的分子标志物.
The incidence of papillary thyroid carcinoma(PTC) is rapidly increasing, and it is urgent to find new biomarkers for treatment and prognosis. The Kinesin family plays an essential role in tumorigenesis and development, as a therapeutic target in a variety of tumors. In this study, based on a public database, the effect of KIF23 on the prognosis of PTC was preliminarily analyzed. It was found that compared with normal tissues,the GEPIA database showed the up-regulated expression of KIF23 gene in thyroid cancer. Two PTC cohorts were downloaded from The Cancer Genome Atlas(TCGA), and survival analysis was performed by KaplanMeier method. It was found that the prognosis of the group with high KIF23 expression was worse than that of the group with low KIF23 expression. Immunohistochemical methods were used to detect the expression and clinicopathological features of KIF23 in 67 thyroid cancer tissues. The results further confirmed the up-regulation of KIF23 expression in thyroid cancer cells. Subsequently, knockdown of KIF23 was performed in thyroid cancer cells, and the expression of KIF23 in thyroid cancer cells was confirmed by RT-qPCR and western blot. The effect of KIF23 on the proliferation of PTC cells was analyzed by colony formation assay and CCK-8 detection.The results showed that the proliferation of thyroid cancer cells was significantly inhibited after KIF23 knockdown expression. The results of this study suggest that the expression of KIF23 is related to the clinicopathological proliferation of thyroid cancer cells. Knockdown of the KIF23 gene can inhibit the pathological proliferation of thyroid cancer cells, which may be a therapeutic target in the future. High expression of KIF23 can serve as a molecular marker for poor prognosis in patients with PTC.
作者
张文瑞
王虹喜
霍磊
Zhang Wenrui;Wang Hongxi;Huo Lei(Department of Surgery,Huanian People's Hospital,Qingyang 745714,China;Department of Traditional Chinese Medicine,Second Afiliated Hospital of Tianjin University of Chinese Medicine,Tianjin 300250,China)
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第2期53-60,共8页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
Supported by the Green Seedling Project of the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine(QMJH202001)。
