摘要
目的评价海马齿状回(DG)钠离子渗漏通道(NALCN)在小鼠社交行为中的作用。方法雄性野生型C57BL/6小鼠39只,6~8周龄,体质量18~22 g。取3只小鼠采用免疫荧光染色法检测海马DG NALCN和神经元核抗原(NeuN)共表达情况。余36只小鼠采用随机数字表法分为2组(n=18):对照组(C组)和NALCN基因敲减组(KO组)。KO组于海马DG注射NALCN腺相关病毒,C组注射对照病毒。病毒注射后3周行三箱社交实验和旷场实验。行为学测试结束后,麻醉下处死小鼠,取海马组织,荧光显微镜下观察病毒注射位置,分别采用Western blot法和RT-PCR法检测海马DG NALCN及其mRNA表达。结果小鼠海马DG NALCN和NeuN存在大量共表达,提示NALCN在海马DG神经元广泛表达。与C组比较,KO组海马DG NALCN及其mRNA表达下调,社交新奇偏好缺失(P<0.05),社交能力及旷场实验各指标差异无统计学意义(P>0.05)。结论海马DG NALCN参与小鼠社交记忆的调控,其表达下调可导致小鼠社交新奇偏好缺失。
Objective To evaluate the role of the sodium leak channel(NALCN)in the hippocampal dentate gyrus(DG)in the social behavior of mice.Methods Thirty-nine male wild-type C57BL/6 mice,aged 6-8 weeks,weighing 18-22 g,were used in this study.Three mice were sacrificed to verify the expression and co-expression of NALCN with neuronal nuclear antigen(NeuN)in the hippocampal DG using the immunofluorescent staining.The remaining 36 mice were divided into 2 groups(n=18 each)by the random number table method:control group(group C)and NALCN gene knockdown group(group KO).NALCN-shRNA virus was injected in group KO,and scrambled-shRNA virus was injected in group C.The three box social test and open field test were performed at 3 weeks after the virus injection.Mice were sacrificed under anesthesia after the behavioral test,hippocampal tissues were collected,and the injection location of the virus was verified with a fluorescence microscope,and the NALCN protein and mRNA expression in the hippocampal DG was detected by Western blot and real-time polymerase chain reaction,respectively.Results NALCN and NeuN co-expressed a lot on the same neuron in the hippocampal DG of mice,indicating that NALCN was widely expressed on the neurons in the hippocampal DG.Compared with group C,the expression of NALCN and mRNA in the hippocampal DG was significantly down-regulated,and the social novelty preference disappeared(P<0.05),and no significant change was found in the social ability and each parameter in the open field test in group KO(P>0.05).Conclusions NALCN in the hippocampal DG is involved in the regulation of social memory in mice,and the down-regulated expression of NALCN can lead to the loss of social novelty preference in mice.
作者
王丽娟
耿晓媛
杨波
张宇
丁宁
孙娜
王涛
赵军博
姜丽华
Wang Lijuan;Geng Xiaoyuan;Yang Bo;Zhang Yu;Ding Ning;Sun Na;Wang Tao;Zhao Junbo;Jiang Lihua(Department of Anesthesiology,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2023年第3期313-316,共4页
Chinese Journal of Anesthesiology
基金
河南省医学科技攻关计划联合共建项目(2018020195)。
关键词
齿状回
钠通道
社交障碍
Dentate gyrus
Sodium channels
Social communication disorder
