摘要
目的:通过研究健脾益气摄血方对免疫性血小板减少症(Immune Thrombocytopenia,ITP)模型小鼠脑肠肽及T细胞相关细胞因子的影响,探讨健脾益气摄血方治疗ITP的效应机制。方法:以被动型免疫造模法建立ITP模型,实验前从30只BALB/c小鼠尾静脉取血,用全自动血细胞计数仪检测血小板计数,按照血小板计数随机分为对照组、ITP组、泼尼松组和健脾益气摄血方中剂量组、高剂量组,每组6只。以酶联免疫吸附试验法检测小鼠脑、结肠及血清中3种脑肠肽和血清、脾T细胞相关细胞因子的水平。结果:与ITP组比较,除血清小鼠垂体腺苷酸环化酶激活多肽(PACAP)外,泼尼松组3种脑肠肽在3种组织均升高;健脾益气摄血方中高剂量均可升高脑血管活性肠肽、结肠食欲刺激素和3种组织的PACAP水平。泼尼松组血清PACAP低于健脾益气摄血方中高剂量组。与ITP组比较,泼尼松降低血清、脾γ干扰素,升高血清、脾白细胞介素-2和脾白细胞介素-4、白细胞介素-10;在血清、脾,健脾益气摄血方中高剂量组可升高白细胞介素-2、白细胞介素-10,降低白细胞介素-17A。处理组间对比,益气摄血方高剂量组血清白细胞介素-2、脾转化生长因子-β1高于泼尼松组;益气摄血方高剂量组脾白细胞介素-17A低于泼尼松组。结论:健脾益气摄血方可从3个机制改善Th1/Th2细胞、Th17细胞/调节性T细胞失衡治疗ITP。1)通过上调脑肠肽水平,改善脑肠轴介导的肠道免疫功能失调,降低Th1细胞、Th17细胞数量,升高Th2细胞、调节性T细胞水平;2)通过抑制Th1细胞分泌γ干扰素和Th17细胞分泌白细胞介素-4、白细胞介素-10,促进Th2细胞分泌白细胞介素-17A和调节性T细胞分泌转化生长因子-β_(1)、白细胞介素-27,逆转Th1/Th2细胞、Th17/调节性T细胞失衡;3)通过上调白细胞介素-2水平改善调节性T细胞缺陷间接恢复Th1/Th2细胞、Th17/调节性T细胞平衡。
Objective:To investigate the effects of Jianpi Yiqi Shexue Prescription on the levels of brain-gut peptides and T cell-related cytokines in a mouse model of immune thrombocytopenia(ITP)and explore its mechanism in the treatment of ITP.Methods:An ITP model was established using the passive immune modeling method.Prior to the experiment,blood was collected from 30 BALB/c mice via tail vein,and platelet counts were measured using an automated blood cell counter.The mice were then randomly divided into a control group,an ITP group,a prednisone group,and low-and high-dose Jianpi Yiqi Shexue Prescription groups,with six mice in each group.The levels of three brain-gut peptides and T cell-related cytokines in the brain,colon,and serum were measured using an enzyme-linked immunosorbent assay(ELISA).Results:Compared with the results in the ITP group,the levels of the three brain-gut peptides increased in the prednisone group except for pituitary adenylate cyclase-activating polypeptide(PACAP)in the serum.The Jianpi Yiqi Shexue Prescription groups were able to increase the levels of vasoactive intestinal peptide in the brain,colon-stimulating peptide,and PACAP in all three tissues.The serum PACAP level in the prednisone group was lower than that in the high-dose Jianpi Yiqi Shexue Prescription group.Compared with the ITP group,the prednisone group showed decreased γ-interferon levels in the serum and spleen and increased interleukin-2,interleukin-4,and interleukin-10 levels in the serum and spleen.In the serum and spleen,the Jianpi Yiqi Shexue Prescription groups showed increased interleukin-2 and interleukin-10 levels and decreased interleukin-17A level.In inter-group comparisons,the high-dose Jianpi Yiqi Shexue Prescription group showed higher serum interleukin-2 and spleen transforming growth factor-β1 levels than the prednisone group,and lower spleen interleukin-17A level than the prednisone group.Conclusion:Jianpi Yiqi Shexue Prescription can improve the imbalance between Th1/Th2 cells and Th17 cells/regulatory T ce
作者
吴洁雅
侯丽
黄子明
何昊
张雅月
WU Jieya;HOU Li;HUANG Ziming;HE Hao;ZHANG Yayue(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Xi′an Medical University,Xi′an 710021,China)
出处
《世界中医药》
CAS
2023年第10期1366-1373,共8页
World Chinese Medicine
基金
国家自然科学基金项目(81703903)。
