摘要
本研究旨在解析对茄雷尔氏菌(Ralstonia solanacearum)具有较强拮抗活性的生防菌株HNU24的基因组特征。采用BGISEQ-500和ONT PromethION平台相结合的策略,对HNU24的全基因组进行了测序、组装、基因预测与功能注释、蛋白质同源分析。遗传进化分析结果表明:HNU24为贝莱斯芽孢杆菌(Bacillus velezensis),其完整基因组序列长度为3932768 bp,GC含量46.48%,编码3822个基因,其中有43个碳水化合物活性酶基因家族。纤维素酶活性实验进一步表明其具有分解纤维素的能力。在HNU24基因组中预测到13个次级代谢产物生物合成基因簇,其中包含表面活性素(surfactin)、泛革素(fengycin)、植物唑霉素(plantazolicin)、大环内酰亚胺(macrolactin)、杆菌烯(bacillaene)、地非西丁(difficidin)、杆菌毒素(bacillibactin)和杆溶菌素(bacilysin)8种已报道的对青枯菌等病原菌有拮抗活性的化合物。5个未能比对到同源基因或相似度较低,可能为未鉴定和报道的新化合物。此外,盆栽试验表明HNU24对番茄具有显著的促生长作用。综上,HNU24可能通过分泌活性化合物来拮抗茄雷尔氏菌并促进番茄生长,是一种潜在的生物肥料菌株,相关结果为进一步开展HNU24在生物防治方面的研究和应用奠定基础。
In order to reveal the genome characteristics of HNU24,which has excellent antagonistic ability to Ralstonia solanacearum,its complete genome was sequenced by the BGISEQ-500 and ONT PromethION technology,and the genome assembly,gene prediction,function annotation,and protein homology analysis also were conducted.Phyloge-netic analysis results demonstrated that HNU24 belonged to Bacillus velezensis.Its completed genomic sequence length was 3932768 bp,GC content was 46.48%,and had 3822 genes.A total of 43 carbohydrate active enzyme gene families were identified in the genome,and the cellulase activity experiment further confirmed that the strain HNU24 had the ability to degrade cellulose.Thirteen clusters of secondary metabolite biosynthesis genes were predicted in genome,including eight compounds:surfactin,fengycin,plantazolicin,butirosin A/butirosin B,macrolactin,bacillaene,difficidin,bacillibactin and bacilysin,which have been reported as the antibacterial components.Five unknown components,which were found no homologous genes or were less similar to the known database sequences,may be new compounds that have not been identified and reported.In addition,greenhouse experiment demonstrated that HNU24 had significant growth promoting effect on tomato plants.Thus,HNU24 may antagonize R.solanacearum and promote tomato growth by secreting active compounds,and it is a potential biofertilizer strain.The relevant results would lay a foundation for further research and application of HNU24 in biological control.
作者
曹秀兰
叶雨婷
马天浩
胡安娜
曹宇
陈鹏泽
伍壮生
李鹏
CAO Xiulan;YE Yuting;MA Tianhao;HU Anna;CAO Yu;CHEN Pengze;WU Zhuangsheng;LI Peng(Hainan Normal University/Key Laboratory of Ministry of Education for Ecology of Tropical Islands/Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province,Haikou,Hainan 571158,China;Vegetable Research Institute,Hainan Academy of Agricultural Sciences,Haikou,Hainan 571100,China)
出处
《热带作物学报》
CSCD
北大核心
2023年第5期905-913,共9页
Chinese Journal of Tropical Crops
基金
海南省重点研发计划项目(No.ZDYF2020080)。
