摘要
目的:研究纳米二氧化硅(SiO_(2))和镉(Cd)联合作用对人脐静脉内皮细胞(HUVEC_(s))的氧化应激和DNA损伤。方法:对照组:不加任何药物;纳米SiO_(2)组:单独加入10μg/ml纳米SiO_(2);CdCl_(2)组:单独加入25μmol/L CdCl_(2);联合组:加入10μg/ml纳米SiO_(2)和25μmol/L CdCl_(2)。通过细胞氧化应激和DNA损伤研究联合毒性,通过析因分析确定纳米SiO_(2)与Cd之间相互作用。结果:单独暴露于纳米SiO_(2)未引起细胞毒性反应,但明显加剧了Cd暴露诱导的氧化应激和DNA损伤,析因分析表明,纳米SiO_(2)和Cd的联合毒性增强是由相加或协同作用引起的。结论:非细胞毒性浓度的纳米SiO_(2)和中等细胞毒性浓度的Cd可通过相加或协同作用增强HUVEC_(s)的氧化应激和DNA损伤。
Objective:To investigate the oxidative stress and DNA damage of human umbilical vein endothelial cells(HUVEC_(s))by the combined action of nano silica(SiO_(2))and cadmium.Methods:Control group:no drug added;nano-SiO_(2)group:separately add 10μg/ml nano-SiO_(2);CdCl_(2)group:adding 25μmol/L CdCl_(2)added;combination group:10μg/ml nano-SiO_(2)and 25μmol/L CdCl_(2).The combined toxicity was studied by cellular oxidative stress and DNA damage,the interaction between nano-SiO_(2)and Cd was determined by factor analysis.Results:Exposure to nano SiO_(2)alone did not cause cytotoxic reactions,but significantly increased the oxidative stress and DNA damage induced by Cd exposure.Factorial analysis showed that the combined toxicity of nano SiO_(2)and Cd was caused by additive or synergistic effects.Conclusion:Non-cytotoxic concentration of nano SiO_(2)and medium cytotoxic concentration of Cd can enhance oxidative stress and DNA damage in HUVEC_(s)by additive or synergistic effect.
作者
赵婧
焦卓亚
王娟
刘璐
骈玉杰
王丹
ZHAO Jing;JIAO Zhuoya;WANG Juan(Department of Preventive Medicine,Fenyang College,Shanxi Medical University,Fenyang City,Shanxi Province 032200)
出处
《医学理论与实践》
2023年第11期1805-1807,1811,共4页
The Journal of Medical Theory and Practice
基金
山西医科大学汾阳学院人才引进基金项目(2017D02)
山西医科大学汾阳学院大学生创新项目(FDC201901)。
