摘要
鸭圆环病毒(DuCV)感染能够引起鸭的免疫抑制,引起多种病原的继发性感染,在我国鸭群中广泛流行。为了解DuCV的遗传变异情况,对采自山东、江苏和贵州3个省份的19个鸭场的样品进行了PCR测定,并对DuCV的ORF-C1基因进行测序和遗传进化分析。PCR测定结果显示,19个鸭场中有9个鸭场的样品为DuCV阳性,总体阳性率为47.4%;ORF-C1基因序列测定结果显示,9株DuCV之间的核苷酸序列和氨基酸序列同源性分别为93.4%~100%和82.2%~100%,与NCBI中已公布的DuCV毒株的核苷酸序列和氨基酸序列同源性分别为77.8%~99.5%和71.5%~-99.6%;遗传进化分析显示,9株DuCV全部属于DuCV-1b亚型,其中有7株在同一分支上,与D11-JW-001等韩国毒株遗传距离较近,另外2株则与山东、广西的毒株遗传距离较近;此外,与已公布的毒株相比,9株DuCV都在158位发生了氨基酸突变,由E/D变为L/P。上述结果表明,DuCV-1仍然为我国鸭群中的优势流行基因型,但病毒的基因也在不断地发生变异,应该对其进行持续监测。
Duck circovirus(DuCV)infection can cause immunosuppression and secondary infection of many kinds of pathogens,and the infection is prevalent in duck flocks in our country.In order to understand the genetic variation of DuCV,19 samples collected in farms in Shandong,Jiangsu and Guizhou provinces were detected by PCR;and the ORF-C1 gene of DuCV was sequenced and analyzed.The results of PCR showed that 9 of the 19 samples were positive for DUCV,with a overall positive rate of 47.4%.The nucleotide sequence and amino acid sequence homology of the 9 DuCV strains were 93.4%-100%and 82.2%-100%,respectively.The nucleotide sequence and amino acid sequence were in 77.8%-99.5%and 71.5%-99.6%homology,respectively,with the published DuCV strains in NCBI.The genetic evolution analysis showed that all 9 strains of DuCV belonged to the DuCV-1b subtype.7 of the strains were in the same branch,which were close to Korean strains such as D11-JW-001,and the other two strains were close to the Shandong and Guangxi strains.In addition,compared with the published strains,all 9 strains of DuCV had amino acid mutation at position 158,which changed from E/D to L/P.These results suggested that DuCV-1 was still the dominant genotype in duck flocks in our country,but the genes of DuCV-1 were constantly changing;therefore,it would be necessary to monitor DuCV-1 continuously.
作者
吕振环
赵莎
卢凤英
杨婧
韩凯凯
黄欣梅
赵冬敏
章丽娇
吴凤瑶
刘宇卓
李银
刘青涛
张小飞
LYU Zhenhuan;ZHAO Sha;LU Fengying;YANG Jing;HAN Kaikai;HUANG Xinmei;ZHAO Dongmin;ZHANG Lijiao;WU Fengyao;LIU Yuzhuo;LI Yin;LIU Qingtao;ZHANG Xiaofei(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《畜牧与兽医》
CAS
北大核心
2023年第5期63-69,共7页
Animal Husbandry & Veterinary Medicine
