摘要
在接种城市污水处理厂A^(2)/O工艺回流污泥的上流式厌氧污泥床(UASB)反应器中,采用逐渐提高进水基质浓度并缩短水力停留时间(HRT)的策略快速启动了厌氧氨氧化(Anammox),对反应器的脱氮效果及启动前后的微生物群落结构差异进行了分析。结果表明,Anammox启动成功仅耗时40 d,氨氮和亚硝态氮去除率均维持在90%以上,最大总氮去除负荷为0.99 kg(/m^(3)·d),ΔNH_(4)^(+)-N∶ΔNO_(2)^(-)-N∶ΔNO_(3)^(-)-N=1∶1.28∶0.26,启动过程中反硝化作用对总氮去除的贡献由原来的83%逐渐降至12%,活性迟滞期末厌氧氨氧化菌的细胞产量达到了(1.37~13.75)×10^(10)个/d;在启动成功初期,厌氧氨氧化菌主要为Candidatus Brocadia(25.14%)和Candidats Jettenia(2.92%),在长时间运行过程中,Candidats Jettenia的丰度逐渐增至12.67%并成为优势菌属。通过构建动力学模型发现,改进的Stover-Kincannon基质去除模型(R^(2)=0.9833)和Grau二级动力学模型(R^(2)=0.9928)适合预测Anammox的脱氮性能和基质去除效率,而Monod模型拟合的R^(2)仅为0.8206,不适合描述不同HRT下UASB-Anammox的脱氮性能。
In an upflow anaerobic sludge bed(UASB)reactor seeded with A^(2)/O return sludge from a municipal sewage treatment plant,Anammox was rapidly started by gradually increasing influent substrate concentration and shortening hydraulic residence time(HRT).The nitrogen removal performance of the reactor and the difference of microbial community structure before and after start-up were analyzed.It took only 40 days to start the reactor successfully.The removal rates of ammonia nitrogen and nitrite nitrogen remained above 90%,the maximum total nitrogen removal load was 0.99 kg/(m^(3)·d),and the ratio of ΔNH_(4)^(+)-N,ΔNO_(2)^(-)-N and ΔNO_(3)^(-)-N was 1∶1.28∶0.26.During the start-up process,the contribution of denitrification to the removal of total nitrogen gradually decreased from 83% to 12%,and the cell yield of Anammox bacteria reached(1.37-13.75)×10^(10) cells/d at the end of the activity retardation period.At the start-up initial stage,the main Anammox bacteria were Candidatus Brocadia(25.14%)and Candidats Jettenia(2.92%).During the long-term operation,the abundance of Candidats Jettenia gradually increased to 12.67% and became the dominant bacteria.The modified Stover-Kincannon substrate removal model(R^(2) of 0.9833)and Grau second-order kinetic model(R^(2) of 0.9928)were suitable for predicting the nitrogen removal performance and substrate removal efficiency of Anammox reactor,while the R^(2) fitted by Monod model was only 0.8206,indicating that it was not suitable to describe the nitrogen removal performance of UASB-Anammox reactor under different HRTs.
作者
王刚
袁林江
王茹
陈希
袁林杰
有小龙
WANG Gang;YUAN Lin-jiang;WANG Ru;CHEN Xi;YUAN Lin-jie;YOU Xiao-long(School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China;Shaanxi Provincial Key Laboratory of Environmental Engineering,Xi’an 710055,China;Northwest China Key Laboratory of Water Resources and Environmental Ecology,Xi’an 710055,China;Zhengzhou Sewage Purification Co.Ltd.,Zhengzhou 450000,China)
出处
《中国给水排水》
CAS
CSCD
北大核心
2023年第9期1-9,共9页
China Water & Wastewater
基金
国家自然科学基金资助项目(51878538)
陕西省教育厅重点实验室研究项目(19JS040)。
