摘要
以不同林龄云南松的根和根际土壤为研究对象,采用苯胺蓝染色-显微镜观察法探究云南松与丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)的关系;采用湿筛倾注-蔗糖离心法分离根际土壤AMF孢子,结合形态学和分子生物学进行鉴定,并分析其物种多样性。结果表明,苯胺蓝压片中未观察到丛枝菌根的特有结构,认为云南松根系与AMF不存在共生关系。根际土壤中共鉴定出7属25种AMF,包括无梗囊霉属6种、近明球囊霉属1种、多样孢囊霉属2种、巨孢囊霉属3种、盾巨孢囊霉属1种、球囊霉属11种、根孢囊霉属1种,其中球囊霉属和无梗囊霉属是云南松根际土壤AMF优势属;云南松林分不同发育阶段根际土壤AMF多样性存在差异,Shannon-Weiner指数、孢子密度和种的丰度为中龄林>幼龄林>成熟林;根际土壤AMF孢子密度、种数和优势种:幼龄林15.15个·g^(-1)、9种、瑞氏无梗囊霉、微白巨孢囊霉、长孢球囊霉,中龄林38.13个·g^(-1)、22种、瑞氏无梗囊霉、珠状巨孢囊霉、疣壁球囊霉,成熟林14.19个·g^(-1)、7种、瑞氏无梗囊霉、细凹无梗囊霉、疣壁球囊霉。
Pinus yunnanensis is a typical ectomycorrhizal fungi species tree.The relationship between arbuscular mycorrhizal fungi(AMF)and different developmental stages of P.yunnanensis forest remains to be studied.The relationship between AMF and the roots and rhizosphere soil of P.yunnanensis of different forest ages was investigated by means of aniline blue staining-microscope observation.AMF spores were isolated from rhizosphere soil by wet sieve pouring-sucrose centrifugation,identified by morphology and molecular biology,and analyzed for species diversity.The results showed that arbuscular mycorrhizal structures were not observed in aniline blue tablets,indicating that there was no symbiotic relationship between the roots of P.yunnanensis and AMF.A total of 25 species of AMF belonging to 7 genera were identified in the rhizosphere soils of P.yunnanensis,including 6 species of Acaulospora,1 species of Claroideoglomus,2 species of Diversispora,3 species of Gigaspora and 1 species of Scutellospora.Glomus 11 species,Rhizophagus 1 species;among them,Glomus and Acaulospora were dominant species of AMF in P.yunnanensis rhizosphere soil.The diversity of AMF in rhizosphere soil of P.yunnanensis forest was different at different development stages.Shannon-weiner index,spore density and species abundance were in the order of middle-aged forest>young forest>mature forest.AMF spore density,species number,and dominant species in rhizosphere soil of different forest ages were as follows:for the young stands,the AMF spore density was 15.15·g^(-1),the species number was 9,and dominant species were A.rehmii,G.albida,and G.dolichosporum.For middle-aged stands,the density was 38.13·g^(-1),species number was 22,and the dominant species were A.rehmii,G.margarita,G.verruculosum.For mature forest,the density was 14.19·g^(-1),with 7 species,and A.rehmii,A.scrobiculata,and G.verruculosum were dominant species.
作者
康定旭
沈德周
刘亭
马莉
伍建榕
马焕成
KANG Ding-xu;SHEN De-zhou;LIU Ting;MA Li;WU Jian-rong;MA Huan-cheng(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China/College of Forestry,Southwest Forestry University,Kunming 650224,Yunnan,China;Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions/College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,Yunnan,China;Dehong State Forestry and Grassland Bureau,Dehong 678400,Yunnan,China)
出处
《西北林学院学报》
CSCD
北大核心
2023年第3期117-122,共6页
Journal of Northwest Forestry University
基金
国家重点研发计划项目(2019YFD1002000)
国家自然科学基金(31560207)
云南省教育厅科学研究基金(2021Y240)。
关键词
云南松
丛枝菌根真菌(AMF)
优势种
真菌多样性
Pinus yunnanensis
arbuscular mycorrhizal fungi(AMF)
dominant species
fungal diversity
