摘要
2-羟基-1-萘甲醛与氨基苯并噻唑缩合获得希夫碱化合物(L_(5)),与CuCl_(2)·2H_(2)O反应得到L_(5)-Cu(Ⅱ)配合物。用荧光光谱研究L_(5)和L_(5)-Cu(Ⅱ)在303,308,313 K下对牛血清白蛋白(BSA)的荧光的影响。结果表明,L_(5)和L_(5)-Cu(Ⅱ)使BSA荧光发生静态猝灭,L_(5)和L_(5)-Cu(Ⅱ)与BSA的结合位点分别为1.5和1,两者与BSA作用的热力学参数为△G<0、△H>0、△S>0,表明其结合过程是自发进行的,主要作用力为疏水力,但L_(5)与BSA结合作用力强于L_(5)-Cu(Ⅱ)。
The Schiff base(L_(5))was synthesized by condensation of 2-hydroxy-1-naphthaldehyde with 2-aminobenzothiazole and reacted with CuCl_(2)·2H_(2)O to form L_(5)-Cu(II)complex.The of L_(5) and L_(5)-Cu(II)bovine serum albumin(BSA)at 303K,308K and 313K were studied by fluorescence spectroscopy.The results showed that the fluorescence of BSA was quenched statically by L_(5) and L_(5)-Cu(II).The binding sites of L_(5) and L_(5)-Cu(II)to BSA were about 1.5 and 1,respectively.The thermodynamic parameters of the interaction between L_(5) and L_(5)-Cu(II)with BSA were△G<0、△H>0、△S>0,which indicating that the combination process is spontaneous and the main action force is hydrophobic force,but the binding force of L_(5) to BSA was stronger than that of L_(5)-Cu(II).
作者
仇继家
张玮玲
袁涛
范歆晨
覃姣兰
Qiu Jijia;Zhang Weiling;Yuan Tao;Fan Yinchen;Qin Jiaolan(Guangxi Key Laboratory of Urban Water Environment,College of Chemistry and Environmental Engineering,Baise University,Baise 533000,China)
出处
《山东化工》
CAS
2023年第6期5-8,12,共5页
Shandong Chemical Industry
基金
国家级大学生创新创业训练计划项目(教高司函[2020]13号,202010609011)
广西省部共建药用资源化学与药物分子工程国家重点实验室开放课题(CMEMR2019-B11)
广西一流学科建设项目资助(农业资源与环境)。
关键词
希夫碱
铜配合物
牛血清白蛋白
荧光光谱
schiff base
copper complex
bovine serum albumin
fluorescence spectra
