摘要
目的基于代谢组学研究灶心土对脾阳虚泄泻模型小鼠的影响。方法将30只昆明小鼠按随机数字表法分为正常对照组、模型组和灶心土组。模型组和灶心土组小鼠采用“饮食失节+苦寒泻下”的方法制备脾阳虚泄泻模型,造模成功后灶心土组灌胃灶心土水煎液12.0 g/kg,正常对照组和模型组灌胃等体积的蒸馏水。1次/d,连续给药7 d。基于超高效液相色谱串联四极杆静电场轨道阱质谱(UPLC-Q-Exactive-MS)技术分析和鉴定小鼠血清代谢物,并结合主成分分析和正交偏最小二乘法判别分析对差异代谢物进行表征,筛选潜在的差异代谢物,并进行KEGG通路富集分析。结果正、负离子模式下分别筛选出110个差异代谢物。灶心土可有效逆转脾阳虚泄泻小鼠的血清代谢紊乱,并可回调12个与脾阳虚泄泻相关的潜在生物标志物水平。KEGG通路富集主要涉及HIF-1信号通路、抗坏血酸和醛糖酸盐代谢通路、血小板激活通路等。结论灶心土可通过下调L-抗坏血酸水平影响HIF-1信号通路、抗坏血酸和醛糖酸盐代谢通路、维生素消化吸收等通路,上调前列腺素G2、H2水平影响血小板激活通路,下调茉莉酸影响α亚麻酸代谢通路,发挥温脾止泻作用。
Objective To study the effects of Terra Flavausta on diarrhea mice with spleen yang deficiency based on metabonomics.Methods Totally 30 mice were divided into normal group,model group and Terra Flavausta group according to random number table method.Mice in the model group and Terra Flavausta group were treated by the method of"diet disorder+clearing fire with herbs bitter in flavour and cold in property"to establish the diarrhea model of spleen yang deficiency.After successful modeling,Terra Flavausta group received Zaoxintu Decoction 12.0 g/kg for gavage,while normal group and model group were given equal volume of distilled water for gavage,for consecutive 7 d.The serum metabolites of each mouse were analyzed and identified based on UPLC-Q-Exective-MS.The differential metabolites were characterized by principal component analysis and orthogonal partial least squares discriminant analysis,and the potential biomakers were screened,and the KEGG pathway enrichment analysis was performed.Results Totally 110 different metabolites were screened under the positive and negative ion mode.Terra Flavausta can effectively reverse the disorder of serum metabolism in diarrhea mice with spleen yang deficiency,and has a significant callback effect on 12 potential biomarkers related to diarrhea with spleen yang deficiency.KEGG pathway enrichment mainly involved HIF-1 signaling pathway,ascorbate and aldarate metabolism,platelet activation,etc.Conclusion Terra Flavausta may play the effect of warming spleen and relieving diarrhea through down-regulation of L-ascorbic acid affecting HIF-1 signal pathway,ascorbic acid and aldose metabolism pathway,vitamin digestion and absorption pathway,up-regulation of prostaglandins G2 and H2 affecting platelet activation pathway,and down-regulation of jasmonic acidαlinolenic acid metabolic pathway.
作者
陈文文
汤丹丹
钟恋
艾莉
吴纯洁
Chen Wenwen;Tang Dandan;Zhong Lian;Ai Li;Wu Chunjie(Department of Pharmacy,Chengdu Women's and Children's Central Hospital,School of Medicine,University of Electronic Science and Technology of China,Chengdu 611731,China;College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)
出处
《国际中医中药杂志》
2023年第5期568-573,共6页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(82204970)
四川省中医药管理局常规专项(2018HJZX004)
四川省科技计划项目(2021YJ0112)。
关键词
灶心土
脾虚泄泻
温脾止泻
代谢组学
Terra Flavausta
Spleen Yang deficiency diarrhea
warming spleen and stopping diarrha
Metabonomics
