摘要
目的考察Fe_(3)O_(4)纳米颗粒在低频振动磁场(low-frequency vibrating magnetic field,VMF)驱动下通过磁场机械力杀伤肿瘤细胞的效果。方法通过共沉淀法合成一种磁性强、具有不规则形貌的立方相Fe_(3)O_(4)纳米颗粒。将其置于本课题组自制的VMF中,研究其介导的磁场机械力对肿瘤细胞的杀伤效果。结果单纯施加VMF对细胞活力无影响;加入Fe_(3)O_(4)纳米颗粒后,细胞活力随VMF处理时间和Fe_(3)O_(4)纳米颗粒浓度的增加而降低,受损细胞释放的乳酸脱氢酶也随磁场处理时间延长而增加。结论不规则形貌Fe_(3)O_(4)纳米颗粒在VMF下可将机械力转移到肿瘤细胞,破坏细胞结构,导致细胞死亡;所采用的VMF装置结构简单、使用安全、操作方便。所采用的磁性粒子及其杀伤肿瘤细胞的方法,有临床转化潜力。
Objective To investigate tumor cell killing effect of superparamagnetic Fe_(3)O_(4)nanoparticles with cubic phase through magneto-mechanical force under a low-frequency vibrating magnetic field(VMF).Methods A kind of strong magnetic and irregular-shaped Fe_(3)O_(4)nanoparticles with cubic phase was synthesized by coprecipitation method.The Fe_(3)O_(4)nanoparticles were exposed to a self-developed VMF and cell killing efficiency of the Fe_(3)O_(4)-mediated magneto-mechanical force was investigated.Results VMF alone had no effects on cell viability.After Fe_(3)O_(4)nanoparticles were added,the cell viability significantly decreased with prolonging the VMF treatment time and increasing the Fe_(3)O_(4)nanoparticle concentration.Lactate dehydrogenase released by damaged cells also increased with prolonging the VMF exposure time.Conclusions The irregular-shaped Fe_(3)O_(4)nanoparticles can transfer magneto-mechanical force to tumor cells under VMF,cause structural damage of cells and result in cell death.The VMF generator developed in this study has simple structure and it is safe for use and convenient for operation.The developed magnetic nanoparticles and the corresponding cancer cell killing technique have the potential for clinical transformation.
作者
李霁
黄雨乔
牛文鑫
储茂泉
LI Ji;HUANG Yuqiao;NIU Wenxin;CHU Maoquan(Research Center for Translational Medicine at Shanghai East Hospital,School of Life Science and Technology,Tongji University,Shanghai 200092,China;Tongji University School of Medicine,Shanghai 200092,China)
出处
《医用生物力学》
CAS
CSCD
北大核心
2023年第2期338-345,共8页
Journal of Medical Biomechanics
基金
生物反应器工程国家重点实验室开放课题(201910132030522125738)。
