摘要
目的:探讨miR-3163调控癌胚抗原相关黏附分子6(CEACAM6)对顺铂耐药胃癌细胞AGS/DDP增殖和凋亡的影响。方法:RT-qPCR和Western blot检测AGS/DDP细胞及亲本细胞株AGS中miR-3163和CEACAM6表达。将AGS/DDP细胞分为DDP+miR-NC、DDP+miR-3163、DDP+si-NC、DDP+si-CEACAM6、DDP+miR-3163+pcDNA和DDP+miR-3163+pcDNACEACAM6组。MTT、流式细胞术分别检测细胞增殖和凋亡。双荧光素酶报告实验和Western blot确定miR-3163与CEACAM6的相互作用。结果:与AGS细胞比较,AGS/DDP细胞miR-3163表达显著降低(0.58±0.06 vs 1.00±0.06),CEACAM6表达显著升高(0.61±0.06 vs 0.24±0.03,P<0.05)。与DDP+miR-NC组比较,DDP+miR-3163组AGS/DDP细胞增殖抑制率[(36.32±3.21)%vs(8.41±0.83)%]、凋亡率[(23.14±2.33)%vs(7.55±0.76)%]显著升高(P<0.05)。与DDP+si-NC组比较,DDP+si-CEACAM6组AGS/DDP细胞增殖抑制率[(31.29±3.18)%vs(7.65±0.77)%]、凋亡率[(19.74±1.83)%vs(6.22±0.63)%]显著升高(P<0.05)。与DDP+miR-3163+pcDNA组比较,DDP+miR-3163+pcDNA-CEACAM6组AGS/DDP细胞增殖抑制率[(18.64±1.58)%vs(39.87±3.77)%]、凋亡率[(10.59±1.04)%vs(24.18±2.43)%]显著降低(P<0.05)。miR-3163靶向负调控CEACAM6表达。结论:miR-3163靶向CEACAM6抑制顺铂耐药胃癌细胞增殖,诱导细胞凋亡,提高其对顺铂的敏感性。
Objective:To investigate effect of miR-3163 on proliferation and apoptosis of gastric cancer cisplatin-resistant cells AGS/DDP by regulating carcinoembryonic antigen cell adhesion molecule 6(CEACAM6).Methods:miR-3163 and CEACAM6 expressions in AGS/DDP cells and parental cell line AGS were detected by RT-qPCR and Western blot.AGS/DDP cells were divided into DDP+miR-NC,DDP+miR-3163,DDP+si-NC,DDP+si-CEACAM6,DDP+miR-3163+pcDNA and DDP+miR-3163+pcDNA-CEACAM6 groups.MTT and flow cytometry were used to detect cell proliferation and apoptosis.Double luciferase reporter assay and Western blot were selected to confirm interaction between miR-3163 and CEACAM6.Results:Compared with AGS cells,expression of miR-3163 in AGS/DDP cells was significantly reduced(0.58±0.06 vs 1.00±0.06),while expression of CEACAM6 was significantly increased(0.61±0.06 vs 0.24±0.03,P<0.05).Compared with DDP+miR-NC group,proliferation inhibition rate[(36.32±3.21)%vs(8.41±0.83)%]and apoptosis rate[(23.14±2.33)%vs(7.55±0.76)%]of AGS/DDP cells in DDP+miR-3163 group were observably increased(P<0.05).Compared with DDP+si-NC group,proliferation inhibition rate[(31.29±3.18)%vs(7.65±0.77)%]and apoptosis rate[(19.74±1.83)%vs(6.22±0.63)%]of AGS/DDP cells in DDP+si-CEACAM6 group were significantly increased(P<0.05).Compared with DDP+miR-3163+pcDNA group,proliferation inhibition[(18.64±1.58)%vs(39.87±3.77)%]rate and apoptosis rate[(10.59±1.04)%vs(24.18±2.43)%]of AGS/DDP cells in DDP+miR-3163+pcDNA-CEACAM6 group were significantly reduced(P<0.05).miR-3163 targeting negatively regulated CEACAM6 expression.Conclusion:miR-3163 inhibits proliferation and induce apoptosis of gastric cancer cisplatin-resistant cells by targeting CEACAM6,and increases its sensitivity to cisplatin.
作者
杨得振
贾勇
董明
侯俊明
刘园蔚
YANG Dezhen;JIA Yong;DONG Ming;HOU Junming;LIU Yuanwei(Department of Surgical Oncology,Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2023年第5期999-1004,共6页
Chinese Journal of Immunology
基金
陕西省重点研发计划项目-社会发展领域(2018ZDXM-SF-001)
陕西省财政厅、陕西省中医药管理局专项资助项目[陕财办社(2016)25号]。
关键词
miR-3163
癌胚抗原相关黏附分子6
胃癌
顺铂耐药
增殖
凋亡
miR-3163
Carcinoembryonic antigen cell adhesion molecule 6
Gastric cancer
Cisplatin-resistance
Proliferation
Apoptosis
