摘要
目的探索新型冠状病毒奥密克戎变异株污染不同物体表面后,不同温度条件下病毒核酸存留时间及变化规律,为环境样本中新冠病毒核酸检测及相关防控策略制定提供依据。方法取实验室保存的新冠肺炎奥密克戎变异株感染者咽拭子灭活样本,污染不锈钢、塑料、木材、纸板及棉布等材质的表面,制成模拟污染样本,放置常温及冷藏环境下,分别在第0 d、1 d、3 d、7 d、14 d和第30 d,每种材料每次取出3件样本,浸泡后提取核酸,使用实时荧光PCR法检测新冠病毒核酸。分析不同类型材料表面污染后病毒核酸回收率、核酸可检出时间和Ct值变化。结果新冠病毒污染后,棉布、塑料和不锈钢核酸的回收率为1.75%~2.90%,木材和纸板较低,分别为0.0021%和0.21%。所有材料模拟样本在第30 d均仍可检测出新冠病毒核酸。不锈钢、木材、纸板及棉布表面病毒核酸Ct值在前7 d内快速上升,随后变化较小。塑料表面病毒核酸Ct值较其它四种材料变化较小。结论新冠病毒污染物体表面后,第30 d仍可检出病毒核酸,且不同性质的物体表面降解模式不同。在日常环境监测中,应结合核酸检测和其他调查手段,综合评估判定污染物品的病毒传播风险。
Objective To explore the retention time and change pattern of the viral RNA of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant on the surfaces of different materials under different temperature,so as to provide a basis for the detection of SARS-CoV-2 on environmental samples and the formulation of relevant prevention and control strategies.Methods The deactivated throat swab samples of coronavirus disease 2019(COVID-19)patients stored in the laboratory were selected and added on the surfaces of stainless steel,plastic,wood,cardboard and cotton cloth to get simulated pollution samples.The samples were placed at room temperature and 4℃.Three samples of each material were taken in batches and soaked on the 0 d,1 d,3 d,7 d,14 d and 30 d for nucleic acid extraction.Real-time RT-PCR was used to detect SARS-CoV-2 viral RNA.The recovery rates,detectable time and changes in Ct values of viral RNA on the surfaces of different materials after contamination were recorded and analyzed.Results After contamination,the recovery rates were 1.75%-2.90%in cotton cloth,plastic and stainless steel,while those were much lower in wood and paperboard.On 30 d after contamination,all material surfaces were positive in SARS-CoV-2 RNA testing.The Ct values of viral RNA on the surface of stainless steel,wood,paperboard and cotton cloth increased rapidly in the first 7 days,and then changed slightly.The Ct values of viral RNA on the surface of plastic changed less comparing to the rest four materials.Conclusions After the virus contamination on the surface of materials,the viral nucleic acid maintained detective for 30 days with different degradation patterns.In routine environment surveillance,nucleic acid detection and other investigation methods should be integrated to comprehensively evaluation and determine the risk of virus transmission by contaminated goods.
作者
梁志超
林长缨
李夫
崔淑娟
张代涛
杨鹏
王全意
潘阳
Liang Zhichao;Lin Changying;Li Fu;Cui Shujuan;Zhang Daitao;Yang Peng;Wang Qaunyi;Pan Yang(Institute for Infectious Disease Prevention and Control,Beijing Center for Disease Prevention and Control,Beijing 100013,China;General Office,Beijing Center for Disease Prevention and Control,Beijing 100013,China)
出处
《国际病毒学杂志》
2023年第2期95-99,共5页
International Journal of Virology
基金
北京市科技计划项目(Z221100007922019)
首都卫生发展科研专项(首发2021-1G-3012)
北京市百千万人才工程项目(2020A051)。
关键词
新型冠状病毒
核酸检测
外环境监测
核酸残留
Severe acute respiratory syndrome coronavirus 2
Nucleic acid detection
Environment surveillance
Nucleic acid residue
