摘要
试验旨在研究平凉红牛CD36基因外显子8(exon 8)多态性及其与肉质性状的关联性。试验利用PCR-SSCP技术、DNA测序技术、实时荧光定量PCR(qPCR)技术进行SNPs位点基因型分型和mRNA表达量测定,分析其与肉质性状的相关性。结果显示,平凉红牛CD36基因exon 8检测出1个SNP位点(g.79219 C>A,aa.Lys>Thr),基因型分为CD和CC型,CD基因型宰前活重、肌内脂肪含量、眼肌面积、胴体重明显高于CC基因型(P<0.05);CD36基因在不同组织器官均表达m RNA,腿肌、背最长肌mRNA表达量较高。研究表明,CD36基因对平凉红牛肉用性状影响较大,可作为改善肉品质的候选分子标记基因。
The experiment was to study exon 8 polymorphism of CD36 gene in Pingliang red cattle and correlation between polymorphism and meat quality traits.The study used PCR-SSCP technology,DNA sequencing technology,realtime quantitative fluorescence PCR(qPCR)technology to determine genotype and mRNA expression of SNPs locus,analyzed its correlation with meat quality traits.The results showed that one SNP locus(g.79219 C>A,aa.Lys>Thr)was detected in CD36 gene exon 8 of Pingliang red cattle,and genotypes were CD and CC.The live weight before slaughter,intramusculus fat content,eye muscle area and carcass weight of CD genotype were significantly higher than those of CC genotype(P<0.05).The mRNA expression of CD36 gene in different tissues and organs was higher in leg muscle and longissimus dorsi muscle.The experiment indicates that CD36 gene has a significant effect on useful traits of Pingliang red cattle,which can be used as a candidate molecular marker gene to improve meat quality.
作者
董和
杨军祥
何茂昌
郭海龙
顾玲荣
桑国俊
DONG He;YANG Jun-xiang;HE Mao-chang;GUO Hai-long;GU Ling-rong;SANG Guo-jun
出处
《饲料研究》
CAS
北大核心
2023年第7期89-93,共5页
Feed Research
基金
甘肃省技术创新引导计划(项目编号:20CX9NA121)
平凉市重点研发计划(项目编号:平科任[2021]21号)
平凉市民生科技计划(项目编号:平科任[2020]12号)
甘肃省农业农村厅现代丝路寒旱农业科技支撑项目(项目编号:GSLK-2022-1)
甘肃省畜牧兽医研究所创新项目(项目编号:GNKJ-2020-8)。
关键词
CD36基因
平凉红牛
多态性
肉用性状
CD36 gene
Pingliang red cattle
polymorphism
meat quality trait
