摘要
背景:肠道菌群失调是结直肠癌(CRC)发生的关键危险因素,近年来中药制剂和益生菌越来越多地应用于CRC的预防工作中。目的:探讨三七总皂苷(PNS)和枯草芽孢杆菌联用对CRC的预防效果。方法:将30只雌性BALB/c小鼠随机分成正常对照组(NC组)、模型组、PNS组、枯草芽孢杆菌组、PNS联合枯草芽孢杆菌组(PaB组)。应用氧化偶氮甲烷(AOM)/葡聚糖硫酸钠(DSS)法构建小鼠CRC模型。记录各组小鼠体质量变化,行疾病活动指数(DAI)评分,评估结直肠长度和肿瘤数量。采用ELISA法测定血清白细胞介素(IL)⁃6和IL⁃10含量。应用16S rRNA测序分析肠道菌群组成。结果:与模型组相比,PaB组小鼠DAI评分明显降低(P<0.001),结直肠长度增加(P<0.001),肿瘤数量明显减少(P<0.001),肿瘤体积明显降低(P<0.01),血清IL⁃6含量明显降低(P<0.0001),IL⁃10含量明显升高(P<0.0001)。肠道菌群测序结果显示,与模型组相比,PaB组Simpson指数明显降低(P<0.05),Shannon指数、Chao指数明显升高(P<0.05),拟杆菌门丰度上升(P<0.01),厚壁菌门丰度下降(P<0.05),螺杆菌属和颤杆菌克丰度明显降低(P<0.05),乳球菌属丰度明显升高(P<0.05)。结论:PNS和枯草芽孢杆菌联用可有效缓解AOM/DSS引起的CRC发生,其作用机制可能与改善肠道微生物群落组成有关。
Background:Dysregulation of intestinal flora is a key risk factor for colorectal cancer(CRC).In recent years,traditional Chinese medicine preparations and probiotics have been increasingly applied in the prevention of CRC.Aims:To investigate the preventive effect of Panax notoginseng saponins(PNS)combined with Bacillus subtilis on CRC.Methods:Thirty female BALB/c mice were randomly divided into normal control group(NC group),model group,PNS group,Bacillus subtilis group and PNS combined with Bacillus subtilis group(PaB group).CRC mice model was constructed by azoxymethane(AOM)/dextran sulfate sodium(DSS)method.During the experiment,the mice were weighed,and disease activity index(DAI)score was evaluated.The length of colorectum and tumor number were measured.Serum interleukin(IL)⁃6 and IL⁃10 contents were determined by ELISA.16S rRNA sequencing was used to analyze the composition of intestinal flora.Results:Compared with model group,DAI score was significantly decreased(P<0.001),colorectal length was significantly increased(P<0.001),number of tumor was significantly decreased(P<0.001),tumor volume was significantly decreased(P<0.01),serum IL⁃6 content was significantly decreased(P<0.0001),and serum IL⁃10 content was significantly increased in PaB group(P<0.0001).The results of intestinal flora sequencing showed that Simpson index was significantly decreased in PaB group than in model group(P<0.05),Shannon index and Chao index were significantly increased(P<0.05),abundance of Bacteroidota was significantly increased(P<0.01),abundances of Firmicutes,Helicobacter and Oscillibacter were significantly decreased(P all<0.05),abundance of Lactococcus was significantly increased(P<0.05).Conclusions:The combination of PNS and Bacillus subtilis can effectively alleviate the occurrence of CRC caused by AOM/DSS,and its mechanism may be related to the improvement of composition of intestinal microbial community.
作者
刘小宾
段毅超
马梦雅
张雪玲
郭汝悦
任雪玲
LIU Xiaobin;DUAN Yichao;MA Mengya;ZHANG Xueling;GUO Ruyue;REN Xueling(School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou,450001)
出处
《胃肠病学》
北大核心
2022年第3期135-143,共9页
Chinese Journal of Gastroenterology
关键词
结直肠肿瘤
三七总皂苷
枯草芽孢杆菌
肠道菌群
Colorectal Neoplasms
Panax notoginseng Saponins
Bacillus subtilis
Intestinal Flora
