摘要
泛素特异蛋白酶(ubiquitin-specific protease,UBP)是去泛素化酶六大家族中最大的,广泛存在于真核生物中,保守性很高。UBP可通过去泛素化作用调节各种生物的生长发育。为了解龙眼(Dimocarpus longan Lour.)DlUBP基因家族的生物学功能及其在龙眼体胚早期发生过程中的作用,基于第3代龙眼基因组数据库与转录组数据库,对DlUBP全基因组进行鉴定,分析其基因结构、蛋白质结构域、启动子顺式元件及构建系统进化树,并对DlUBP基因在脱落酸处理和干旱胁迫处理下的表达情况进行分析。结果显示,DlUBP家族共有18个成员,分为10个亚家族,其中最大的亚家族有4个成员;各成员的氨基酸数为369~1117个,内含子数为2~31个;在DlUBP启动子序列中存在大量光响应元件、厌氧诱导响应元件、激素响应元件,随着处理时间的增加,在脱落酸处理下,多数DlUBP基因的表达量呈降低趋势,在干旱胁迫处理下,有不少DlUBP基因的表达量升高,推测DlUBP基因在龙眼体胚早期参与脱落酸的调控并对干旱胁迫有应激反应。
Ubiquitin-specific protease(UBP)is the largest among the six families of deubiquitinating enzymes.It is widely found in eukaryotes and is highly conserved.They regulate the growth and development of various organisms through deubiquitination.In order to understand the biological function of DlUBP gene family and its role during somatic embryogenesis of Dimocarpus longan Lour.In this study,we performed genome-wide identification of DlUBP gene family members;analysed their genes structure,protein structure,promoter cis-acting elements and constructed phylogenetic tree based on the third-generation longan genome database and transcriptome database;then analyzed the expression of DlUBP under ABA treatment and drought stress.The results showed that the DlUBP gene family contains 18 members,which are divided into 10 subfamilies,the largest subfamily has four members;the number of amino acids are between 369-1117,and the number of introns are between 2-31;There are a large number of light response elements,anaerobic induction response elements,and hormone response elements in the DlUBP promoter sequence with a prolonged procecsing time,the expression level of many DlUBP members showed a decreasing trend under abscisic acid treatment,and the expression level of many DlUBP genes increased under drought stress.It is speculated that the DlUBP gene family participates in the regulation of abscisic acid during somatic embryogenesis of longan and responses to drought stress.
作者
童宁
张春渝
许小琼
陈晓慧
申序
赖钟雄
TONG Ning;ZHANG Chunyu;XU Xiaoqiong;CHEN Xiaohui;SHEN Xu;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《浙江大学学报（理学版）》
CAS
CSCD
北大核心
2023年第3期367-377,390,共12页
Journal of Zhejiang University（Science Edition）
基金
国家自然科学基金资助项目(31572088)
福建省高原学科建设经费项目(102/71201801101)
福建农林大学科技创新专项基金项目(CXZX2020027A).
关键词
龙眼
UBP基因
成员鉴定
表达情况
Dimocarpus longan
UBP gene
genome-wide identification
expression
