摘要
目的探究西洛他唑对糖尿病小鼠肠黏膜屏障的影响。方法从GEO数据库中下载GSE142153数据集,分析其差异基因变化。将8周龄的雄性db/db小鼠以及对照m/m小鼠随机分为m/m+溶剂组、m/m+西洛他唑组、db/db+溶剂组、db/db+西洛他唑组。各组小鼠分别用西洛他唑和相应溶剂灌胃4周。干预结束后,检测血清sCD40L水平及肠道CD40表达,以小鼠肠道通透性、含水量、菌落数、紧密连接蛋白含量等指标评估小鼠肠黏膜屏障功能。结果糖尿病患者差异基因富集在血小板活化、内皮细胞屏障功能等通路中。db/db小鼠血清sCD40L水平明显升高,肠道组织CD40表达、通透性、含水量、菌落数明显上升,紧密连接蛋白表达下降。西洛他唑干预db/db小鼠后,血清sCD40L、肠道CD40水平明显下降,肠黏膜屏障功能明显改善。结论西洛他唑可以改善糖尿病小鼠肠黏膜屏障损伤,其保护作用可能与抑制血小板活化相关。
Objective To explore the effect of cilostazol on intestinal barrier function in type 2 diabetes(T2DM).Methods The GSE142153 dataset was downloaded from GEO database to analyze gene changes in diabetic patients.Eight-week-old male db/db mice and control m/m mice were randomly divided into m/m+cmc,m/m+cilo,db/db+cmc,and db/db+cilo groups.Mice in different groups were given cilostazol and corresponding solvents for 4 weeks.We detected the levels of serum sCD40L and the expression of CD40 in intestinal tissue,and evaluated the mice’s intestinal barrier function by examining intestinal permeability,water content,bacterial number,and tight junction protein expression in different groups.Results Differential expressed genes were enriched in platelet activation and endothelial barrier function pathways in diabetic patients.Compared with those in the control group,the levels of serum sCD40L in db/db diabetic mice elevated significantly,and the CD40 expression,permeability,water content and bacterial number in intestinal tissue increased obviously,while the expression of tight junction protein decreased.Cilostazol treatment in diabetic mice decreased the levels of serum sCD40L and CD40,and alleviated significantly the intestinal barrier dysfunction.Conclusion Cilostazol attenuated the damage of intestinal barrier function in T2DM,and its protective effect may be related to the inhibition of platelet activation in diabetic mice.
作者
宋平义
蒋世秋
胡娟
檀佳璐
杨岚
王强
SONG Pingyi;JIANG Shiqiu;HU Juan;TAN Jialu;YANG Lan;WANG Qiang(Department of Anesthesiology&Center for Brain Science,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2023年第3期403-408,共6页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.82274290)。
