摘要
【目的】克隆桃热激转录因子PpHSF18基因,分析其在2种树型桃中的表达,探究其在分枝角度形成中的功能。【方法】测定一年生普通型桃大久保和柱型桃洒红龙柱枝条不同生长时期分枝角度,并分析11个桃PpHSFAs基因在2种树型中的表达;克隆PpHSF18基因,构建PpHSF18基因的过表达载体,并进行拟南芥遗传转化,探究其对拟南芥株型和分枝角度的影响。【结果】柱型桃品种洒红龙柱枝条分枝角度显著小于普通型桃大久保,大久保桃分枝角度随着枝条生长逐渐增大,而洒红龙柱桃分枝角度变化不明显;11个桃A类HSF转录因子家族基因在2种树型桃茎尖中的表达呈3种趋势,其中PpHSF18与PpLAZY1呈相同表达趋势,即在柱型桃中表达量显著高于普通型桃,且与水稻OsHSFA2D同源性最高;PpHSF18三个转基因株系分枝角度均小于野生型拟南芥分枝角度,表明PpHSF18参与植物分枝角度的形成。【结论】过表达PpHSF18导致转基因拟南芥分枝角度变小,为进一步解析PpHSF18在桃分枝角度形成中的作用提供理论依据。
【Objective】Peach(Prunus persica L.)is an important economical fruit and is popular with consumers.In peach,the branch angle,as an important agronomic trait of tree architecture,affects fruit yield and quality,adaptive capacity to environment,and competitive capacity.The pillar peach,as one of the special germplasm resources,shows smaller branch angle and fewer secondary branches than the standard peach,which is an ideal tree architecture for implementing easy pruning and mechanized har-vesting strategies.Heat shock factors(HSFs),especially for class A,play a vital role in not only biotic or abiotic stress but also normal plant growth and development,especially in plant tree architecture.However,PpHSFAs have not been studied in peach,especially the role in regulating branch angle.The objectives of this study aimed to screen and validate the candidate PpHSFAs function that might partici-pate in formation of branch angle.【Methods】A pillar peach(Sahonglongzhu,S)and a standard peach(Okubo,O)with one-year old seedlings were selected for measuring branch angle,respectively.The shoot-tip of these two cultivars was taken for RNA-seq.The expression pattern of 11 PpHSFAs,PpTAC1 and PpLAZY1 were detected and the heatmap was plotted by TBtools software.The homology of PpHSFAs with OsHSFA2D was detected using Blastp search and the gene with the highest expected value was selected as the candidate gene.PpHSF18 was selected and might be involved in the forma-tion of branch angle,which showed a high similarity with OsHSFA2D.Then,PpHSF18 was cloned by PCR and transformed in pSAK277 vector,and promoted by 35S promoter,using the restriction en-zymes HindⅢand XbaⅠ.The recombinant vector was transformed into GV3101 strain.The pSAK277-35S::PpHSF18 was transformed into Arabidopsis thaliana using floral dip method by Agro-bacterium-mediated transformation.The positive plants were identified by PCR and the relative expres-sion levels of PpHSF18 in transgenic lines were detected by quantitative real-time PCR(qRT-PCR).The Arabid
作者
李天豪
张杰
高红珠
魏鹏程
郑先波
连晓东
王小贝
张海朋
程钧
王伟
谭彬
冯建灿
LI Tianhao;ZHANG Jie;GAO Hongzhu;WEI Pengcheng;ZHENG Xianbo;LIAN Xiaodong;WANG Xiaobei;ZHANG Haipeng;CHENG Jun;WANG Wei;TAN Bin;FENG Jiancan(College of Horticulture,Henan Agricultural University,Zhengzhou 450002,Henan,China;Henan Key Laboratory Fruit and Cucurbit Biology,Zhengzhou 450002,Henan,China)
出处
《果树学报》
CAS
CSCD
北大核心
2023年第5期852-860,共9页
Journal of Fruit Science
基金
河南省重大公益科技专项(201300110500)
河南省高等学校青年骨干教师培养计划项目(2019GGJS044)
河南省杰出外籍科学家工作室(GZS2020007)
河南省现代农业产业技术体系建设专项(HARS-22-09-G1)
河南省青年科学基金(30602313)。
关键词
桃
热激转录因子
分枝角度
柱型
Peach(Prunus persica)
Heat shock transcription factor
Branch angle
Pillar
