摘要
【目的】探索RNA结合蛋白PTBP1与p-AXL在骨肉瘤中的表达情况及其临床病理联系,并进一步探讨PTBP1/p-AXL共表达在骨肉瘤中的预后评估意义。【方法】应用GEO和Target数据库分析PTBP1与AXL在骨肉瘤和正常组织中的表达差异及PTBP1的预后价值。收集2016年3月至2020年10月中山大学第一附属医院76例骨肉瘤和37例非恶性骨组织(骨痂、骨纤维结构不良或骨样骨瘤)及其病例信息,应用免疫组化法检测PTBP1和p-AXL蛋白的表达情况并行统计学分析。【结果】GEO数据库分析结果显示PTBP1和AXL在骨肉瘤组织的表达具有高于正常组织的趋势,但尚未达到统计学意义;Target数据分析结果显示PTBP1高表达组骨肉瘤患者总生存期(OS)与无进展生存期(PFS)均短于低表达组,但差异尚未达统计学意义(P=0.064;P=0.134)。免疫组化结果显示PTBP1和p-AXL蛋白的表达在骨肉瘤组织中显著高于非恶性骨组织;p-AXL阳性表达率与肺转移相关(P=0.025);Kaplan-Meier分析发现肺转移、复发、PTBP1表达及PTBP1/p-AXL共表达等因素与骨肉瘤患者不良总生存期相关;且多变量Cox回归分析显示肺转移(P<0.0001)、PTBP1表达阳性(P=0.041)是骨肉瘤患者总生存期(OS)独立危险因素;PTBP1/p-AXL共表达患者的OS(P=0.017)和PFS(P=0.043)均低于非PTBP1/p-AXL共表达患者。【结论】PTBP1、p-AXL在骨肉瘤中高表达,PTBP1与p-AXL共表达与患者预后差相关,且PTBP1可作为骨肉瘤患者独立预后指标。
【Objective】To explore the co-expression of PTBP1 and p-AXL in osteosarcoma and its clinicopathological significance for prognosis evaluation.【Methods】The expression of PTBP1 and AXL and their prognostic value in osteosarcoma were analyzed by GEO and Target data.Paraffin biopsy specimens and clinical information from 76 cases of osteosarcoma and 37 cases of non-malignant bone tissue(callus,osteofibrous dysplasia and osteoid ostema)were obtained from the First Affiliated Hospital of Sun Yat-sen University from March 2016 to October 2020.The expressions of PTBP1 and p-AXL proteins in osteosarcoma were detected by immunohistochemistry.【Results】GEO database showed that the expression levels of PTBP and AXL in osteosarcoma tumor group were higher than those in normal tissues,but did not reach statistical significance.Target database showed that the high expression of PTBP 1 had shorter Overall survival(OS)and Progression free survival(PFS)than low PTBP 1 expression,but did not reach statistical significance(P=0.064;P=0.134).Immuno-histochemical staining included 76 cases of osteosarcoma and 37 cases of non-malignant bone tissue.The expression rate of PTBP 1 and p-AXL protein in osteosarcoma tissues was higher than that in non-malignant bone tissue.The expression of p-AXL is correlated with lung metastasis(P=0.025).Kaplan-Meier analysis showed that lung metastasis,recurrence,PT-BP 1 expression,coexpression of PTBP 1/p-AXL influence the prognosis of patients in OS.Multivariate Cox regression analysis showed that lung metastasis(P<0.0001)and positive expression of PTBP 1(P=0.041)were independent risk factors for osteosarcoma patients in OS.Co-expression of PTBP 1 and p-AXL had shorter OS(P=0.017)and PFS(P=0.043)than non-coexpression osteosarcoma patients.【Conclusions】PTBP 1 and p-AXL were highly expressed in osteosarcoma tis-sues.The co-expression of PTBP 1 and p-AXL was associated with poor prognosis of patients,and PTBP 1 could be used as an independent prognostic indicator of patients with osteosarcoma.
作者
李湘湘
徐雯莉
卢正宇
何安芳
王震
刘妮
乐雨婷
彭挺生
LI Xiang-xiang;XU Wen-li;LU Zheng-yu;HE An-fang;WANG Zhen;LIU Ni;LE Yu-ting;PENG Ting-sheng(Department of Pathology,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2023年第3期446-455,共10页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金(81872175)。
