摘要
湖羊是江浙地区常见的绵羊品种之一,繁殖性能优越,肉质鲜嫩,营养丰富,为了进一步探索湖羊的繁殖潜力,采用CIDR+PMSG+PG+FSH法对湖羊进行同期发情和超数排卵,并在此基础上对发情母羊进行腹腔镜子宫角输精;采用相同输精密度、不同输精时间(撤栓后48、54、60 h)分别对发情母羊进行腹腔镜子宫角授精,通过冲胚进行卵子受精率和可用胚胎率检查,探究对湖羊进行腹腔镜子宫角输精时的最适输精密度与最适输精时间。结果表明,输精密度为2×10^(8)个/mL组的卵子受精率和可用胚胎率显著低于其余4组(P<0.05),其余4组差异不显著(P>0.05);撤栓后48 h输精组与撤栓后54 h输精组可用胚胎率差异不显著(P>0.05),但均高于撤栓后60 h,且差异显著(P<0.05);不同输精密度的鲜精以及不同输精时间均会对湖羊卵子受精率和可用胚胎率产生一定的影响,输精密度为3×10^(8)个/mL可以作为湖羊鲜精输精的最低有效密度,撤栓后48~54 h输精可以作为湖羊腹腔镜子宫角输精的最佳时间。
Hu Sheep is one of the common sheep breeds in Jiangsu and Zhejiang regions, with excellent reproductive performance, fresh and tender meat and rich nutrition. In order to further explore the reproductive potential of Hu sheep, CIDR+PMSG+PG+FSH method was used to conduct simultaneous estrus and superovulation of Hu sheep, and on this basis, laparoscopic uterine horn insemination was performed on the estrous ewes;Laparoscopic uterine horn insemination was performed on estrous ewes using the same insemination density and different insemination times (48, 54, and 60 hours after thrombolysis removal). The fertilization rate and available embryo rate were examined by embryo flushing to explore the optimal infusion precision and time for laparoscopic uterine horn insemination in Hu sheep. The results showed that the fertilization rate and available embryo rate of the 2×10^(8) pieces/mL group were significantly lower than those of the other 4 groups ( P< 0.05), while the other 4 groups had no significant difference ( P> 0.05);There was no significant difference in the available embryo rate between the 48 h after thrombectomy and the 54 h after thrombectomy groups ( P> 0.05), but both were higher than those at 60 h after thrombectomy, and the difference was significant ( P< 0.05);fresh semen with different precision and different insemination time have certain influence on fertilization rate and available embryo rate of Hu sheep eggs, 3×10^(8) pieces/mL could be used as the lowest effective density of fresh semen insemination in Hu sheep. 48-54 hours after embolectomy could be the best time for laparoscopic uterine horn insemination in Hu sheep.
作者
高峰
李心海
GAO Feng;LI Xin-hai(Xuzhou Institute of Biotechnology,Xuzhou,Jiangsu 221000)
出处
《安徽农业科学》
CAS
2023年第8期96-98,108,共4页
Journal of Anhui Agricultural Sciences
基金
徐州市科技项目(KC20048)。
关键词
输精密度
输精时间
冲胚
胚胎质量
绵羊
Infusion precision
Insemination time
Flushing embryo
Embryo quality
Sheep
