摘要
以分离自青贮燕麦中的植物乳杆菌L-Z1为研究对象,以黄曲霉为指示菌,通过测定L-Z1对黄曲霉生物被膜、细胞膜通透性、磷代谢和呼吸系统酶活性的影响,从而初步阐明L-Z1对黄曲霉的抑菌作用和抑菌机制。结果表明:加入植物乳杆菌L-Z1后,黄曲霉抑菌圈直径为(17.15±0.23)mm(7.8 mm被含),生物被膜抑制率高达56.6%,黄曲霉的可溶性总糖和可溶性蛋白含量增加,磷代谢和呼吸系统酶的活性均降低,证明植物乳杆菌L-Z1对黄曲霉具有较强抑菌性,抑菌机制是减弱黄曲霉的细胞呼吸作用及破坏细胞膜。
In this study,Lactobacillus plantarum L-Z1 isolated from silage oats was used as the research object,and Aspergillus flavus was used as the indicator bacteria.Therefore,the antibacterial effect and antibacterial mechanism of L-Z1 on Aspergillus flavus were preliminarily clarified.The results showed that:after adding Lactobacillus plantarum L-Z1,the inhibition zone cliameter of Aspergillus flavus was(17.15±0.23)mm(7.8 mm was contained),the biofilm inhibition rate was up to 56.6%,the content of soluble total sugar and soluble protein of Aspergillus flavus increased,phosphorus metabolism and respiratory enzymes activities were decreased,which proved that Lactobacillus plantarum L-Z1 had strong antibacterial activity against Aspergillus flavus.The bacteriostatic mechanism is to weaken the cellular respiration and destroy the cell membrane of Aspergillus flavus.
作者
张艳芳
胡炜东
曹晓娟
武俊英
ZHANG Yanfang;HU Weidong;CAO Xiaojuan;WU Junying(College of Vocational Technical,Inner Mongolia Agricultural University,Baotou 014109,China)
出处
《食品科技》
CAS
北大核心
2023年第3期24-29,共6页
Food Science and Technology
基金
内蒙古自治区自然科学基金项目(2020MS03052)。
关键词
植物乳杆菌
黄曲霉
抑菌机制
呼吸作用
细胞膜
Lactobacillus plantarum
Aspergillus flavus
antibacterial mechanism
respiration
cell membrane
