摘要
为分析不同品种公猪精液品质及受精能力的差异,测定临沂某猪场的沂蒙黑猪、江泉黑猪和杜洛克3个品种12~36月龄公猪精液品质及受精能力的各项指标。采用单因素方差分析和多重比较方法,比较不同品种各项指标均数的差异性,校正品种效应后,计算各项指标间的相关系数。结果表明,江泉黑猪与沂蒙黑猪在采精量、精子密度、有效精子总数和稀释份数上无显著差异,但均显著高于杜洛克;江泉黑猪与杜洛克在精子活力上无显著差异,但均显著高于沂蒙黑猪;3个品种公猪的精液在稀释后,每份精液的有效精子数无显著差异,受胎率也无显著差异;江泉黑猪在采精量、有效精子总数、稀释份数上表现出较高的杂种优势,杂种优势率在3.8%~5.5%。相关性分析结果表明,原精的有效精子总数与稀释份数呈显著正相关(r=0.99,P<0.01),采精量与原精的有效精子总数和稀释份数均呈显著正相关(r=0.92,P<0.01);采精量与原精的精子密度、稀释后的精子密度、直线运动精子数、有效精子数存在中等程度的负遗传相关(r=-0.37~-0.21),但对受胎率无显著影响(r=-0.06);除了原精的精子密度与受胎率呈中等程度的正相关外(r=0.28,P<0.01),稀释前和稀释后各精液品质指标与受胎率均呈弱相关。综上,在人工授精过程中精液稀释有利于保证母畜受孕,江泉黑猪在采精量、有效精子总数等指标上存在一定程度的杂种优势且优于父本杜洛克。
To analyze the differences in semen quality and fertilization ability of different breeds of boars,various indicators of semen quality and fertilization ability were measured for Yimeng black pigs,Jiangquan black pigs,and Duroc pigs aged 12 to 36 months at a pig farm in Linyi.We used one‑way ANOVA and multiple comparison methods to compare the differences in various indicators among different breeds.The breed effect was corrected and the correlation coefficients between various indicators were calculated.The results showed no significant difference between Jiangquan black pigs and Yimeng black pigs in terms of ejaculate volume,sperm density,total effective sperm count,and dilution ratio,but both were significantly higher than Duroc.There was no significant difference between Jiangquan black pigs and Duroc regarding sperm motility,but both were significantly higher than Yimeng black pigs.There was no significant difference in the total effective sperm count per unit volume after dilution and fertilization rate among the three breeds of boars.Jiangquan black pigs exhibited heterosis in terms of ejaculate volume,total effective sperm count,and dilution ratio,with a heterosis rate of 3.8%—5.5%.The correlation analysis showed that the total effective sperm count of the raw semen was significantly positively correlated with the dilution ratio(r=0.99,P<0.01),and the ejaculate volume was significantly positively correlated with the total effective sperm count and dilution ratio of the raw semen(r=0.92,P<0.01).The ejaculate volume was moderately negatively genetically correlated with sperm density,diluted sperm density,straight‑line movement sperm count,and total effective sperm count of the raw semen(r=-0.37—-0.21).However,it did not significantly affect the final fertilization rate(r=-0.06).Except the moderate positive correlation between the sperm density of the raw semen and fertilization rate(r=0.28,P<0.01),the pre‑diluted and post‑diluted semen quality indicators were weakly correlated with the fertiliza
作者
杨锁州
王文文
王丹
宁超
王杨
李倩
董立才
公维龙
李鑫
唐辉
YANG Suozhou;WANG Wenwen;WANG Dan;NING Chao;WANG Yang;LI Qian;DONG Licai;GONG Weilong;LI Xin;TANG Hui(College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China;Shandong Futong Agriculture and Animal Husbandry Industry Development Limited Company,Linyi 276014,China)
出处
《河南农业科学》
北大核心
2023年第4期137-142,共6页
Journal of Henan Agricultural Sciences
基金
“十四五”国家重点研发计划项目(2021YFD1301203)
山东省农业良种工程项目(2020LZGC012)。
关键词
沂蒙黑猪
江泉黑猪
杜洛克
精液品质
受精能力
Yimeng black pig
Jiangquan black pig
Duroc
Semen quality
Fertilization capacity
