摘要
目的探讨肿瘤患者血清脂质代谢与免疫功能的相关性。方法选取2018年7月至2021年4月在复旦大学附属金山医院肿瘤科就诊的214例肿瘤患者为研究组,105例非肿瘤人群为对照组。采用液相色谱质谱分析(LC-MS)技术对两组人群血清进行靶向代谢组学检测,筛选出差异脂质分子。采用流式细胞技术检测两组人群外周血免疫细胞含量、免疫细胞因子水平,并进行差异脂质分子与免疫功能指标的相关性分析。结果共筛选出37个差异代谢产物,包括磷脂酰胆碱(PC)、溶血磷脂酰乙醇胺(LPE)、磷脂酰乙醇胺(PE)、胆固醇酯(CE)、二酰甘油(DAG)、鞘磷脂(SM)和三酰甘油(TAG)。相关性分析结果显示,肿瘤患者血清中PC、PE、CE、DAG、SM和TAG类脂质分子与中央记忆CD8^(+)T细胞、Th2细胞、调节性T细胞、记忆调节性T细胞和CD16^(+)NK细胞的数量正相关,与Th1细胞和幼稚调节性T细胞数量负相关。LPE类脂质分子则与效应CD8^(+)T细胞和Th1细胞正相关,与Th2细胞负相关(P<0.05)。结论肿瘤患者血清中PC和CE显著上调,LPE、PE、DAG、SM和TAG显著下调,这些脂质分子与淋巴细胞亚群数量和免疫细胞因子水平显著相关。
Objective To explore the correlation between serum lipid metabolism and immune function in tumor patients.Methods A total of 214 cancer patients admitted to the Department of Oncology,Jinshan Hospital,Fudan University from July 2018 to April 2021 were selected as the research group and 105 non-cancer patients as the control group.Liquid mass spectrometry(LC-MS)was used to detect the targeted metabolomics of plasma from the two groups,and the differential lipid molecules were screened out.Flow cytometry was used to detect the levels of immune cells and cytokines in peripheral blood of the two groups,and the correlation between different lipid molecules and immune function indexes was analyzed.Results A total of 37 metabolites were screened between the two groups,including phosphatidylcholine(PC),lyso phosphatidyl ethanolamine(LPE),phosphatidyl ethanolaminece(PE),diacylglycerol(DAG),sphingomyelin(SM)and triacylglycerol(TAG).Correlation analysis showed that PC,PE,CE,DAG,SM and TAG lipid molecules in serum were positively correlated with the number of central memory CD8^(+)T cells,Th2 cells,Treg cells,memory Treg cell and CD16^(+)NK cell,and negatively correlated with Th1 cell and naive Treg cell.LPE lipid molecules were positively correlated with effector CD8^(+)T cells and Th1 cell,but negatively correlated with Th2 cell(P<0.05).Conclusions PC and CE are significantly up-regulated in the serum of tumor patients,while LPE,PE,DAG,SM and TAG are significantly down-regulated.These lipid molecules are significantly correlated with the number of lymphocyte subsets and the level of immune cytokines.
作者
李许硕
杨志诚
王克
杨莹
何晓萍
邹欣
程韵枫
乔田奎
武多娇
LI Xu-shuo;YANG Zhi-cheng;WANG Ke;YANG Ying;HE Xiao-ping;ZOU Xin;CHENG Yun-feng;QIAO Tian-kui;WU Duo-jiao(Center for Tumor Diagnosis&Therapy,Jinshan Hospital,Fudan University,Shanghai 201508,China)
出处
《中国临床医学》
2023年第2期293-300,共8页
Chinese Journal of Clinical Medicine
基金
上海市金山区科学技术创新资金项目(2021-3-03)
复旦大学附属金山医院青年启动基金(JYQN-LC-202102)。
