摘要
目的基于核转录因子-κB(nuclear factor kappa-B,NF-κB)/白介素-1β(interleukin-1β,IL-1β)通路探讨金银花总皂苷对铅中毒大鼠的保肝作用。方法清洁级SD大鼠100只分为正常对照组、模型对照组、金银花总皂苷低、中、高剂量组,除正常对照组外,其余各组大鼠每日腹腔注射醋酸铅溶液(10 mg/kg),持续4周;期间,金银花总皂苷低、中、高剂量组大鼠每日予以相应剂量的金银花总皂苷灌胃,正常对照组、模型对照组给予等体积的生理盐水灌胃。试验结束后获取大鼠血清及肝脏,测定肝/体比值、肝铅含量、血清丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)、γ-谷氨酰转移酶(gamma-glutamyltransferase,GGT)水平、肝丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平,反转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)法及蛋白印迹法测定肝NF-κB和IL-1β水平。结果与正常对照组比较,模型对照组体质量、SOD、GSH-Px水平降低,肝/体比值、肝铅含量、血清AST[(256.32±16.24)vs(46.81±15.24)μ/L]、ALT[(354.32±18.54)vs(35.36±15.85)μ/L]、GGT水平[(5.64±0.31)vs(0.94±0.24)μ/L]、肝MDA水平[(654.49±16.29)vs(266.54±15.52)ng/g]、肝NF-κB、IL-1βmRNA蛋白表达水平[0.82±0.28 vs 3.61±0.23,0.48±0.29 vs 3.71±0.32,0.49±0.12 vs 1.69±0.11,0.38±0.16 vs 1.98±0.14]升高(P<0.05);与模型对照组比较,金银花总皂苷低、中、高剂量组体质量、SOD、GSH-Px水平降低升高,肝/体比值、肝铅含量、血清AST[(88.32±15.74)vs(135.20±10.8)、(186.28±12.27)、(256.32±16.24)μ/L]、ALT[(154.68±19.78)vs(204.28±20.47)、(257.68±19.44)、(354.32±18.54)μ/L]、GGT水平[(2.23±0.27)vs(3.27±0.34)、(4.73±0.27)、(5.64±0.31)μ/L]、肝MDA水平[(358.58±15.41)vs(485.52±10.59)、(588.69±12.53)、(654.49±16.29)ng/g]、肝NF-κB、IL-1βmRNA�
Objective To investigate the hepatoprotective effect of total saponins of honeysuckle on lead poisoning rats based on nuclear factor kappa-B(NF-κB)/interleukin-1β(IL-1β)pathway.Methods 100 clean SD rats were divided into normal control group,model control group,low,medium and high dose groups of total honeysuckle saponins.Except the normal control group,the other groups were injected with lead acetate solution(10 mg/kg)for four weeks.During this period,the rats in the low,medium and high dose groups of total honeysuckle saponins were given the corresponding doses of total honeysuckle saponins by gavage every day,and the normal control group and the model control group were given the same volume of normal saline by gavage.After the test,the rat serum and liver were obtained,and the liver/body ratio,liver lead content,serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),gamma-glutamyltransferase(GGT),malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)levels were determined.The reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting were used to determine liver NF-κB and IL-1βlevels.Results Compared with the normal control group,the body weight,SOD and GSH-Px levels of the model control group decreased,liver/body ratio,liver lead content,serum AST[(256.32±16.24)vs(46.81±15.24)μ/L],ALT[(354.32±18.54)vs(35.36±15.85)μ/L],GGT level[(5.64±0.31)vs(0.94±0.24)μ/L],liver MDA level[(654.49±16.29)vs(266.54±15.52)ng/g],liver NF-κB and IL-1βmRNA protein expression levels[0.82±0.28 vs 3.61±0.23,0.48±0.29 vs 3.71±0.32,0.49±0.12 vs 1.69±0.11,0.38±0.16 vs 1.98±0.14]increased(P<0.05).Compared with the model control group,the body weight,SOD,GSH-Px levels in the low,medium and high dose groups of total saponins of honeysuckle decreased,serum AST[(88.32±15.74)vs(135.20±10.8),(186.28±12.27),(256.32±16.24)μ/L],ALT[(154.68±19.78)vs(204.28±20.47),(257.68±19.44),(354.32±18.54)μ/L],GGT level[(2.23±0.27)vs(3.27±0.34),(4.73±0.27),(5.64±0.31)
作者
黄华
郑冲
张凯
HUANG Hua;ZHENG Chong;ZHANG Kai(Occupational Health and Radiological Health Department,Jiangjin District Center for Disease Control and Prevention,Chongqing,402260,China;Toxicology Department,Guizhou Provincial Center for Disease Control and Prevention,Guiyang Guizhou,550000,China)
出处
《职业与健康》
CAS
2023年第5期620-625,共6页
Occupation and Health
基金
贵州省疾病预防控制中心青年基金(2019-E2-7青)。
