摘要
比较刺梨汁(Rosa roxburghii Tratt juice,RRTJ)、石榴汁(Pomegranate juice,PJ)以及蓝莓汁(Blueberry juice,BJ)的活性成分含量以及抗氧化活性,探究三种果汁对葡聚糖硫酸钠盐(Dextran sulfate sodium,DSS)诱导人正常结肠上皮细胞NCM460氧化损伤的保护作用。结果表明,三种果汁中共同含有的生物活性成分有28种,其中刺梨汁的总多酚、总黄酮含量显著高于石榴汁和蓝莓汁(P<0.05),分别为22.77和12.04 mg/mL;同时,刺梨汁对ABTS+·、DPPH·的清除能力显著高于石榴汁和蓝莓汁(P<0.05),半数清除率(Half scavenging rate,IC50)分别为4.00±0.32和10.03±0.51μL/mL;Pearson相关性分析表明果汁的总多酚含量与ABTS+·清除能力呈正相关(P<0.05)。此外,刺梨汁缓解DSS诱导NCM460细胞氧化损伤的能力最强,2μL/mL刺梨汁即能使氧化损伤的NCM460细胞活力恢复到与对照组相当的水平,降低DSS引起的细胞中活性氧(Reactive oxygen species,ROS)水平。实验表明果汁中的总多酚含量与抗氧化能力呈正相关;刺梨汁的总多酚和总黄酮含量高于蓝莓汁和石榴汁,抗氧化活性和缓解DSS诱导NCM460细胞氧化损伤的能力最强,具有深入研究开发的潜力。
To compare the contents of active ingredients and antioxidant activity of Rosa roxburghii Tratt juice(RRTJ),pomegranate juice(PJ)and blueberry juice(BJ)and investigate the protective effects of three kinds of fruit juices against dextran sulfate sodium(DSS)-induced oxidative damage in human normal colonic epithelial cells NCM460.There were 28 bioactive components in the three juices.The total polyphenols and total flavonoids of RRTJ were significantly higher than those in the PJ and BJ(P<0.05),22.77 and 12.04 mg/mL,respectively.Meanwhile,the ABTS+·and DPPH·scavenging abilities of the juice were significantly higher than those in the PJ and BJ(P<0.05),and the half scavenging rate(IC50)was 4.00±0.32 and 10.03±0.51μL/mL,respectively.Pearson correlation analysis indicated that the total polyphenol content of the juice was positively correlated with the ABTS+·scavenging ability(P<0.05).In addition,RRTJ had the strongest ability to alleviate DSS-induced oxidative damage in NCM460 cells,and 2μL/mL RRTJ could restore the viability of oxidatively damaged NCM460 cells to a level comparable to the control and reduce the level of reactive oxygen species(ROS)in DSSinduced cells.The results showed that the content of total polyphenols in fruit juice was positively correlated with antioxidant capacity.The content of total polyphenols in juice was positively correlated with antioxidant capacity,and the antioxidant activity and the ability to alleviate DSS-induced oxidative damage in NCM460 cells were the strongest in vitro,which had the potential for in-depth research and development.
作者
周婷
吴雪莉
李星洁
唐克纯
武首薰
黄孝懿
康宇鸿
夏锐
王礼群
阴文娅
ZHOU Ting;WU Xueli;LI Xingjie;TANG Kechun;WU Shouxun;HUANG Xiaoyi;KANG Yuhong;XIA Rui;WANG Liqun;YIN Wenya(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu 610041,China;Sichuan Institute of Product Quality Supervision and Inspection,Chengdu 610100,China)
出处
《食品工业科技》
CAS
北大核心
2023年第10期353-361,共9页
Science and Technology of Food Industry
基金
四川省自然科学基金(2022NSFSC0587)。
关键词
刺梨汁
石榴汁
蓝莓汁
总多酚
总黄酮
抗氧化活性
氧化损伤
Rosa roxburghii Tratt juice
pomegranate juice
blueberry juice
total polyphenols
total flavonoids
antioxidant activity
oxidative damage
