摘要
目的了解上饶市献血人群戊型肝炎病毒(HEV)感染情况及分布特征。方法留取上饶市中心血站2021年2月—2022年4月46837例无偿献血者的血液样本,采用酶联免疫吸附试验(ELISA)检测HEV-IgM抗体,HEV-IgM阳性样本进行逆转录聚合酶链反应(RT-PCR)检测HEV-RNA;采用整群抽样方法,从46837例献血者中抽出8449例留取样本进行HEV-IgG抗体检测。结果本地区献血人群HEV-IgM和IgG阳性率分别为0.25%(117/46837)、20.52%(1734/8449),检出HEV-RNA阳性1例,HEV-RNA总体阳性率为0.002%(1/46837)。HEV-IgM阳性率在不同性别、血型、学历和献血次数间差异无统计学意义(P>0.05);随着年龄增长,阳性率显著升高(P<0.05)。HEV-IgG阳性率在不同性别和血型间差异无统计学意义(P>0.05);随着年龄增长,献血次数的累积以及学历下降,阳性率均显著升高(P<0.05)。HEV-IgM阳性结果S/CO值大部分在1.00~3.00区间(87.18%);HEV-IgG阳性结果S/CO值分布跨度较大,以6.01~53.00区间(47.58%)为主。结论上饶市献血人群HEV感染率较低,但HEV经输血传播仍有一定风险,本次研究为血液筛查策略提供了数据支持。
Objective To understand the infection and distribution characteristics of hepatitis E virus(HEV)among blood donors in Shangrao City.Methods The blood samples of 46,837 unpaid blood donors from February 2021 to April 2022 were collected.Enzyme-linked immunosorbent assay(ELISA)was used to detect HEV-IgM antibodies,and HEV-IgM positive samples were subjected to reverse transcription polymerase chain analysis.Reaction(RT-PCR)was used to detect HEV RNA;using cluster sampling method,8449 samples were drawn from 46837 blood donors for HEV-IgG antibody detection.Results The positive rates of HEV-IgM and IgG among blood donors in this area were 0.25%(117/46837)and 20.52%(1734/8449),respectively.One case was positive for HEV RNA,and the overall positive rate for HEV RNA was 0.002%(1/46837).There was no significant difference in the positive rate of HEV-IgM among different genders,blood types,educational backgrounds and blood donation times(P>0.05).The positive rate increased significantly with age(P<0.05).There was no significant difference in the positive rate of HEV-IgG among different genders and blood types(P>0.05);with the increase of age,the accumulation of blood donation times and the decrease of educational background,the positive rate increased significantly(P<0.05).Most of the S/CO values of HEV-IgM positive results were in the range of 1.00 to 3.00(87.18%);the distribution of S/CO values of HEV-IgG positive results was large,mainly in the range of 6.01 to 53.00(47.58%).Conclusion The infection rate of HEV among blood donors in Shangrao City is low,but there is still a certain risk of HEV transmission through blood transfusion.This study provides data support for the blood screening strategy.
作者
余司华
蔡县成
吕年德
何辉
刘芳菲
徐申
王茜
Yu Sihua;Cai Xiancheng;Lv Niande(The Center Blood Station of Shangrao City,Shangrao,Jiangxi 334000)
出处
《基层医学论坛》
2023年第13期119-122,共4页
The Medical Forum
基金
上饶市科技计划项目(2021CZDX40)。
