摘要
目的:通过网络药理学研究方法探讨生蒲黄-墨旱莲药对治疗糖尿病视网膜病变(Diabetic retinopathy,DR)的作用机制。方法:通过中药药理数据库和分析平台(TCMSP)检索药物的活性成分和靶点;在GeneCards、DrugBank和OMIM数据库检索糖尿病视网膜病变相关靶点;利用Venny工具找出疾病与药物的交集靶点;通过Sting数据库对交集靶点进行分析;结果导入Cytoscape 3.9.1软件进行拓扑异构分析;筛选出核心靶点并绘制蛋白质-蛋白质相互作用网络图。将交集靶点录入Metascape进行基因本体论(GO)功能分析和京都基因与基因组百科全书(KEGG)通路分析,得到潜在作用通路。结果:(1)药物活性成分及靶点:通过TCMSP数据库筛选出生蒲黄-墨旱莲活性成分17个、作用靶点222个。(2)疾病靶点:经GeneCards、DrugBank、OMIM数据库筛选去重后获得相关的疾病靶点4005个,将药物靶点与疾病靶点交集后共获得108个共有靶点。(3)核心靶点:通过Cytoscape 3.9.1软件筛选出核心靶点为丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1)、血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)A、白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-1β(Interleukin-1β,IL-1β)等。(4)KEGG和GO富集分析:GO富集分析确定了1772个条目(P<0.01),KEGG通路富集筛选得到195条信号通路(P<0.01),主要有肿瘤坏死因子(Tumor Necrosis Factor,TNF)信号通路、缺氧诱导因子(Hypoxia-inducible Factor,HIF)-1α信号通路、癌症信号通路、糖尿病并发症中的晚期糖基化终末产物(Advanced Glycation End Products,AGE)-晚期糖基化终末产物受体(AGE Receptor,RAGE)信号通路、VEGF信号通路等。结论:生蒲黄-墨旱莲治疗DR的作用机制可能与抗炎、抗氧化、抑制新生血管生成等作用有关。
Objective:To preliminarily explore the underlying mechanism of anti diabetic retinopathy(DR)with Sheng Puhuang(Pollen Typnae)combined with Mohanlian(Herba Ecliptae)based on network pharmacology analysis.Methods:The active components and target information of drugs were retrieved through TCMSP database.And the DR-related targets were retrieved from GeneCards,Drugbank and OMIM databases.Then the intersection targets of disease and drugs were found by Venny tool.The intersection targets were analyzed through Sting database,and the results were imported into Cytoscape 3.9.1 to carry out topological heterogeneity analysis,select the core targets,and draw the PPI network diagram.The intersection target was inputted into Metascape for gene ontology(GO)function analysis and Kyoto Encyclopedia of genes and genomes(KEGG)pathway analysis to obtain the potential pathway.Results:(1)Active components and targets of drugs:A total of 17 active components and 222 action targets of Sheng Puhuang and Mohanlian were screened through TCMSP database.(2)Disease targets:A total of 4005 related disease targets were obtained after screening and de-duplication by GeneCards,Drugbank and OMIM databases.A total of 108 common targets were obtained after the intersection of drug targets and disease targets.(3)Core target:Through Cytoscape 39.1,the core targets AKT1,VEGFA,IL-6,IL-1βand so on were screened out.(4)KEGG and GO enrichment analysis:A total of 1772 items were determined by GO enrichment analysis(P<0.01).A total of 195 signal pathways were obtained by KEGG pathway enrichment and screening(P<0.01),mainly including TNF signal pathway and HIF-1αsignaling pathways,cancer signaling pathways,AGE-RAGE signaling pathways in diabetes complications and VEGF signaling pathways.Conclusion:The mechanism of Sheng Puhuang and Mohanlian in the treatment of DR may be related to anti-inflammatory,antioxidant and inhibition of angiogenesis.
出处
《中医临床研究》
2023年第5期35-41,共7页
Clinical Journal Of Chinese Medicine
关键词
生蒲黄
墨旱莲
糖尿病视网膜病变
网络药理学
靶点预测
Sheng Puhuang
Mohanlian
Diabetic retinopathy
Network pharmacology
Target prediction
