摘要
目的探讨干酪乳杆菌-山药复合发酵剂对溃疡性结肠炎(UC)小鼠肠道菌群的调节及其抗炎作用。方法将干酪乳杆菌(SJP6841L2)与山药粉末混合发酵,制成干酪乳杆菌-山药复合发酵剂。将60只小鼠随机分为空白对照组、UC模型组、山药对照组、低剂量复合发酵剂组、中剂量复合发酵剂组、高剂量复合发酵剂组,每组10只。UC模型组、山药对照组、低剂量复合发酵剂组、中剂量复合发酵剂组、高剂量复合发酵剂组小鼠给予2,4,6-三硝基苯磺酸诱导,制备UC模型;正常对照组小鼠经肛门注射体积分数50%乙醇。造模成功后,正常对照组、UC模型组小鼠每天经口灌胃生理盐水(10 mL·kg^(-1)),山药对照组小鼠每天经口给予200 mg·kg^(-1)无发酵山药制剂灌胃,低剂量复合发酵剂组、中剂量复合发酵剂组、高剂量复合发酵剂组每天经口分别给予50、100、200 mg·kg^(-1)干酪乳杆菌-山药复合发酵剂灌胃,持续10 d。干预10 d后,观察各组小鼠黏液脓血便、腹泻等一般症状;处死各组小鼠,取结肠,检测结肠的长度、厚度、重量,观察各组小鼠结肠组织水肿情况;应用苏木精-伊红染色观察各组小鼠结肠组织病理学变化,紫外分光光度法检测各组小鼠结肠组织中髓过氧化酶(MPO)活性,酶联免疫吸附法检测各组小鼠结肠组织中白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、血红素氧合酶-1(HO-1)和核转录因子-κB(NF-κB)的表达水平,Western blot法检测各组小鼠结肠组织中肠道上调密封蛋白1(claudin 1)和闭锁小带蛋白-1(ZO-1)表达,细菌生理生化鉴定法检测结肠肠道中双歧杆菌、乳酸杆菌、拟杆菌、肠杆菌数量,气相色谱法检测各组小鼠结肠肠道中乙酸、丙酸、丁酸水平。结果与单一山药干预比较,干酪乳杆菌-山药复合发酵剂能显著改善UC小鼠结肠组织的长度、厚度、重量(P<0.05),及黏液脓血便、腹泻等肠炎症状,减少UC小�
Objective To explore the regulation effects of Lactobacillus casei-yam compound starter on intestinal flora and its anti-inflammatory in mice with ulcerative colitis(UC).Methods Lactobacillus casei(SJP6841L2)and yam powder were mixed and fermented to produce Lactobacillus casei-yam compound starter.A total of 60 mice were randomly divided into blank control group,UC model group,yam control group,low-dose compound starter group,medium-dose compound starter group and high-dose compound starter group,with 10 mice in each group.The mice in the UC model group,yam control group,low-dose compound starter group,medium-dose compound starter group and high-dose compound starter group were induced by 2,4,6-trinitrobenzene sulfonic acid to prepare UC model;the mice in the normal control group were injected with volume fraction 50%ethanol through anus.After successful modeling,the mice in the normal control group and UC model group were administrated normal saline(10 mL·kg^(-1))by oral gavage every day;the mice in the yam control group were administrated 200 mg·kg^(-1) non-fermented yam preparation by oral gavage every day;and the mice in the low-dose compound starter group,the medium-dose compound starter group and the high-dose compound starter group were administrated 50,100 and 200 mg·kg^(-1) Lactobacillus casei-yam compound starter by oral gavage every day,respectively,lasted for 10 days.After treatment for 10 days,the general symptoms including mucopurulent bloody stool and diarrhea of mice in each group were observed;the mice in each group were killed,the colon was taken,and the length,thickness and weight of the colon were measured,the edema of the colon tissue was observed;the pathological changes of the colon tissue of mice in each group was observed by hematoxylin-eosin staining,the activity of myeloperoxidase(MPO)in colon tissue of mice in each group was detected by ultraviolet spectrophotometry,and the expressions of interleukin-8(IL-8),tumor necrosis factor-α(TNF-α),heme oxygenase-1(HO-1)and nuclear factor-
作者
朱瑜
郑志
朱绍辉
候栋
陈璇
權英珠
朴丙熙
姜林娟
ZHU Yu;ZHENG Zhi;ZHU Shaohui;HOU Dong;CHEN Xuan;KWON Youngjoo;PARK Byounghee;JIANG Linjuan(Xinxiang Key Laboratory of Applied Microbial Technology,School of Public Health,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Hanling Medical Technology Research Institute Co.,Nanjing 211100,Jiangsu Province,China;Xinxiang Key Laboratory of Medicine,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China;School of Pharmacy,Ewha Woman′s University,Seoul 04393,Republic of Korea;Raphagen Company Limited,Seoul 04393,Republic of Korea)
出处
《新乡医学院学报》
CAS
2023年第5期401-410,共10页
Journal of Xinxiang Medical University
基金
河南省高等学校重点科研项目(编号:22B320007)
河南省引进国外智力专项(编号:HNGD2022066)
新乡医学院海外归国博士启动金(编号:505098)。
关键词
益生菌
干酪乳杆菌
山药
溃疡性结肠炎
抗炎
肠道菌群
probiotics
Lactobacillus casei
yam
ulcerative colitis
anti-inflammatory
intestinal flora
