摘要
目的观察胃癌患者外周血循环肿瘤细胞(circulating tumor cell,CTC)程序性死亡配体-1(programmed death-ligand 1,PD-L1)表达变化,探讨其与胃癌临床病理特征的关系。方法行胃癌根治术的72例胃癌患者为胃癌组,同期20例体检健康者为对照组,术前采集2组外周血,应用Cyttel检测技术完成CTC富集及检测,比较2组CTC阳性率。取胃癌组46例CTC阳性患者的CTC,检测PD-L1阳性表达情况,并分为PD-L1阳性组34例和PD-L1阴性组12例,比较2组的临床病理特征。结果胃癌组外周血CTC阳性率(63.89%)高于对照组(5.00%)(χ^(2)=21.722,P<0.001)。46例CTC阳性胃癌患者外周血CTC PD-L1阳性表达34例(73.91%),阴性表达12例(26.09%)。PD-L1阳性组临床分期Ⅲ~Ⅳ期(89.29%)、浸润深度T_(3-4)(82.86%)比率均高于PD-L1阴性组(10.71%、17.14%)(χ^(2)=8.770,P=0.003;χ^(2)=6.072,P=0.014),年龄≥60岁、男性、腺癌、肿瘤直径>5 cm、组织病理分级低分化、肿瘤位于贲门及有神经侵犯、脉管侵犯、淋巴结转移、远处转移者比率与PD-L1阴性组比较差异均无统计学意义(P>0.05)。结论胃癌患者外周血CTC阳性率增高,CTC阳性胃癌患者CTC PD-L1高表达表明肿瘤临床分期晚、浸润程度深。
Objective To observe the expression of programmed death-ligand 1(PD-L1)in peripheral blood circulating tumor cells(CTC)of patients with gastric cancer,and to investigate its relationship with the clinicopathological characteristics of gastric cancer.Methods The peripheral blood was collected in 72 patients with gastric cancer(gastric cancer group)and 20 healthy volunteers(control group).CTC enrichment and detection were done by using Cytel detection technology,and the positive rate of CTC was compared between two groups.The positive expression of PD-L1 was detected in CTC-positive gastric cancer patients,by which 46 patients were divided into PD-L1-positive group(n=34)and PD-L1-negative group(n=12),and the clinicopathological characteristics were compared between two groups.Results The positive rate of CTC in peripheral blood was higher in gastric cancer group(63.89%)than that in control group(5.00%)(χ^(2)=21.722,P<0.001).In 46 CTC-positive gastric cancer patients,PD-L1 was positively expressed in 34 patients(73.91%)and negatively expressed in 12 patients(26.09%).The percentages of patients with clinical stagesⅢ-Ⅳand T_(3-4)were higher in PD-L1-positive group(89.29%,82.86%)than those in PD-L1-negative group(10.71%,17.14%)(χ^(2)=8.770,P=0.003;χ^(2)=6.072,P=0.014),and there were no significant differences in the percentages of patients with age≥60 years,male,adenocarcinoma,tumor diameter>5 cm,poorly differentiated histopathological grade,tumor at the cardia,nerve invasion,vascular invasion,lymph node metastasis,and distant metastasis between two groups(P>0.05).Conclusion The positive rate of CTC increases in peripheral blood of patients with gastric cancer,and the high expression of CTC PD-L1in patients with CTC-positive gastric cancer indicates a late clinical stage and deep degree of invasion.
作者
王涛
杨敬舒
张璐璐
王尚鑫
陈紫来
郭振
孙培春
WANG Tao;YANG Jing-shu;ZHANG Lu-lu;WANG Shang-xin;CHEN Zi-lai;GUO Zhen;SUN Pei-chun(Department of Gastroenterological Surgery,Zhengzhou University People's Hospital,Henan Provincial People's Hospital,Zhengzhou,Henan 450003,China;Department of Gastroenterological Surgery,Henan University People's Hospital,Henan Provincial People's Hospital,Zhengzhou,Henan 450003,China)
出处
《中华实用诊断与治疗杂志》
2023年第3期261-264,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
河南省医学科技攻关计划省部共建重点项目(SBGJ202102028)。
关键词
胃癌
循环肿瘤细胞
程序性死亡配体-1
临床病理特征
gastric cancer
circulating tumor cell
programmed death-ligand l
clinicopathological characteristics
