摘要
CRISPR-Cas9技术属于新型基因编辑技术中的一种,相比ZFNs和TALENs具有操作简单、成本低廉和编辑率高等特点,被广泛应用于生命科学和医学领域。在植物科学研究中,CRISPR技术可以高效地编辑目标基因来构建突变体,从而为植物基因功能研究提供材料,具有十分重要的意义。然而高等生物基因功能冗余及其复杂互作关系,导致研究中常需构建双/多突变体,而传统的遗传杂交存在工作量大、周期长等问题。CRISPR-Cas9技术是近年来广泛用于基因编辑的技术,其能同时对基因组中多个靶位点进行编辑,极大地缩短了研究中高阶突变体获取的周期和难度。本研究介绍了几种基于CRISPR-Cas9的多基因编辑策略,以期为植物多基因编辑领域的技术整合提供有效信息。
CRISPR-Cas9 system is one of the new gene editing technologies.Compared with ZFNs and TALENs,CRISPR-Cas9 system is characterized by simple operation,low cost and higher editing rate,and has been widely used in the field of life science and medicine.In plant science research,CRISPR system can efficiently edit target genes to generate mutants,which are invaluable genetic materials for gene functional studies.However,due to the functional redundancy of genes and their complex interactions in higher organisms,double/multiple mutants are usually needed to be constructed in research,and the traditional genetic hybridization cannot serve scientific research well due to its labor intensive and time consuming.In recent years,with the development of CRISPR-Cas9 technology,multiple target sites in genome can be edited simutaneously,greatly shortening the period and difficulty of obtaining multiple mutants in research.In this study,several CRISPR-Cas9-based strategies for multiplex gene editing are reviewed in order to provide effective information for technical integration in the field of plant multiplex gene editing.
作者
郎楠
梁洛瑜
汪军丽
武磊
Lang Nan;Liang Luoyu;Wang Junli;Wu Lei(Key Laboratory of Cellular Activity and Adversity Adaptation of Ministry of Education,Lanzhou University,Lanzhou,730000)
出处
《分子植物育种》
CAS
北大核心
2023年第8期2665-2670,共6页
Molecular Plant Breeding
基金
兰州大学中央高校基本科研业务费专项(lzujbky-2020-33)
国家级大学生创新创业训练计划项目(20211-0730142)共同资助。
