摘要
自河北衡水市选择6个黄顶菊Flaveria bidentis入侵样地,采集根区土壤和根系样品,分离丛枝菌根(AM)真菌,测定其物种多样性状况;于盆栽条件下评估上述试验确定的优势AM真菌对黄顶菊生长发育的影响;采用隔网分室培养系统测定促生效应最大的AM真菌优势种组合对黄顶菊与伴生植物三叶草Trifolium repens间碳氮运转分配的影响。从根区土壤和根内分别鉴定到AM真菌8属26种和5属7种,其中光壁无梗囊霉Acaulospora laevis、根内根孢囊霉Rhizophagus intraradices、摩西斗管囊霉Funneliformis mosseae和变形球囊霉Glomus versiforme为根区土壤的优势种;接种F.mosseae+R.intraradices+A.laevis处理对黄顶菊的促生效应最大。分室培养系统中分别对黄顶菊和三叶草接种AM真菌和施加15N或13C处理后,黄顶菊茎叶和根系15N和13C丰度均明显高于三叶草的。结论认为,入侵样地黄顶菊根区有丰富的AM真菌物种多样性,其中,优势种F.mosseae+R.intraradices+A.laevis组合有助于黄顶菊从三叶草中夺取碳氮养分,显著提高入侵寄主竞争力,改善营养状况,促进其入侵建群。
Root zone soil and root samples of Flaveria bidentis were collected from the six invasion sites in Hengshui of Hebei Province and arbuscular mycorrhizal(AM)fungi were isolated to investigate their species diversity status.The effects of the dominant AM fungi on the growth and development of F.bidentis were evaluated under pot culture conditions,and the effects of the fungi on transportation and distribution of carbon and nitrogen between F.bidentis and its companion plant Trifolium repens were also determined with compartmentalized culture system.In total,26 species of AM fungi belonging to 8 genera and 7 species belonging to 5 genera were identified from the soil and roots of the invasive plant,respectively.Among them,Acaulospora laevis,Rhizophagus intraradices,Funneliformis mosseae,and Glomus versiforme were dominant for root zone soil.Inoculation of F.mosseae+R.intraradices+A.laevis had the greatest growth promoting effect on F.bidentis.After being inoculated with AM fungi and treated with 15N or 13C in a compartment culture system,the abundance of 15N and 13C in the stem,leaf and root of F.bidentis were significantly higher than that of T.repens.It was concluded that there was a rich diversity of AM fungi of the invasive plant,and the combination treatment of F.mosseae+R.intraradices+A.laevis could help the invasive plant F.bidentis in transporting carbon and nitrogen from its companion plant T.repens,significantly improving the invasive plant competitiveness and nutritional status,and promoting its invasion and colony establishment.
作者
胡学意
吴海英
祝诚尚
李伟
郭霄
李敏
郭绍霞
HU Xueyi;WU Haiying;ZHU Chengshang;LI Wei;GUO Xiao;LI Min;GUO Shaoxia(College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao 266109,Shandong,China;Institute of Mycorrhizal Biotechnology,Qingdao Agricultural University,Qingdao 266109,Shandong,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2023年第3期692-706,共15页
Mycosystema
关键词
黄顶菊
丛枝菌根
生长发育
养分传递
Flaveria bidentis
arbuscular mycorrhizal fungi
growth and development
nutrient transmission
