摘要
为评估草地贪夜蛾从玉米转移为害水稻的风险,将室内以玉米叶继代饲养10代以上的草地贪夜蛾初孵幼虫分别饲喂玉米叶和水稻叶,其后代为CnC1和CnR1;CnR1组用水稻叶继代饲养8代后再分为2组,一组继续饲喂水稻叶并繁殖1代(CnR8R1),另一组取食玉米并繁殖1代(CnR8C1),分别观察、记录这4个种群幼虫的历期,计算其存活率、繁殖力,测定其营养效应和中肠消化酶活性。结果表明:CnC1组的幼虫历期最短(17.46 d),而CnR1幼虫历期最长(23.24 d),这两者间差异显著;CnR1组存活率显著低于其他处理组;CnC1组单雌产卵量最高(693粒),CnR1组的单雌产卵量最低(384粒);CnR1组和CnR8R1组幼虫的相对取食量均显著低于CnC1组和CnR8C1组的;4个处理组间幼虫食物利用率和转化率的差异不显著;寄主转换过程中幼虫中肠的胃蛋酶活性的差异显著,但脂肪酶和淀粉酶活性的差异不显著。由此可见,从幼虫的存活率、繁殖力、营养效应、中肠消化酶活性综合来看,取食水稻叶的种群重新转移为害玉米后不会影响其在玉米上的种群增长速率;水稻虽不是草地贪夜蛾的最适寄主,但草地贪夜蛾从玉米转移至水稻后仍具有逐渐适应取食并为害水稻的潜力。
To evaluate the risk of Spodoptera frugiperda(S.frugiperda)switching from corn to rice,after 10 generations of successive feeding on corn leaves in the laboratory,the newly hatched S.frugiperda larvae were fed corn and rice,respectively,and the following generations,CnC1 and CnR1,were produced.The CnR1 was fed with rice leaves for 8 generations and then divided into 2 groups.One group continued to feed on rice and reproduced 1 generation(CnR8R1),and the other group fed on corn and reproduced 1 generation(CnR8C1).Then,the duration of larvae in CnC1,CnR1,CnR8R1 and CnR8C1 treatment groups was recorded,the survival rate and fecundity were calculated,and nutritional effect and digestive enzyme activity were also detected.These results indicate that larval duration of CnC1 was the shortest(17.46 d)and of CnR1 was the longest(23.24 d),with significant difference between the two groups.The survival rate of CnR1 was significantly lower than those of other treatments.CnC1 group had the most eggs laid per female(693 eggs),whereas CnR1 had the fewest eggs laid per female(384 eggs).Additionally,the relative consumption rate of CnR1 and CnR8R1 larvae was significantly lower than those of CnC1 and CnR8C1.The efficiency of conversion of ingested and digested food of larvae showed no significant difference among the four treatment groups.There was significant difference in pepsin activity of larval midgut during host transition period,but there were no significant difference in lipase and amylase activities.Therefore,it was determined that the population growth rate on corn was unaffected when the population on rice was host re-switching to corn,based on larval survival rate,fecundity,nutritional effect and digestive enzyme activity.Although rice is not the most suitable host for S.frugiperda,it still has the potential to adapt to feeding on and damaging rice after it switched from corn to rice.
作者
杨露
汤勇
张政兵
林宇丰
贺华良
丁文兵
邱林
李有志
YANG Lu;TANG Yong;ZHANG Zhengbing;LIN Yufeng;HE Hualiang;DING Wenbing;QIU Lin;LI Youzhi(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China;Agricultural and RuralBureau of Changde City,Changde,Hunan 415000,China;Plant Protection Station of Hunan Province,Changsha,Hunan 410005,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第2期190-194,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科学技术厅重点研发计划项目(2020NK2034)
湖南省科学技术厅项目(2021ZK4010)
长沙市科学技术局自然科学基金项目(kq2202226)。
关键词
草地贪夜蛾
玉米
水稻
寄主转换
适应性
营养效应
消化酶
Spodoptera frugiperda
corn
rice
host switch
adaptation
nutritional effect
digestive enzyme
