摘要
目的:研究对比酶联免疫吸附法(ELISA法)及聚合酶链反应法(PCR法)在生殖器疱疹病毒抗原检测中的应用价值。方法:随机选取2017年1月至2022年10月在临沂市皮肤病医院进行诊治的疑似生殖器疱疹患者87例,采集患者标本并分别应用ELISA法及PCR法进行单纯疱疹病毒抗原检测。以病毒培养法检测结果作为金标准,分析ELISA法及PCR法在生殖器疱疹病毒抗原检测中的应用效果。结果:两种检测方法对HSV-1感染、HSV-2感染、混合感染的检出率以及总阳性检出率的差异均无统计学意义(P>0.05)。以病毒培养法检测结果为金标准,ELISA法检测与PCR法检测诊断生殖器疱疹灵敏度、准确度、特异度、阳性预测值、阴性预测值的差异均无统计学意义(P>0.05)。结论:ELISA法及PCR法在生殖器疱疹病毒抗原检测中的应用价值均较高,但是ELISA法具有经济实用、操作简单等特点,更加适合基层医院推广和应用。
Objective:To compare the application value of enzyme-linked immunosorbent assay(ELISA)and polymerase chain reaction(PCR)in the detection of genital herpes virus antigen.Methods:A total of 87 patients with suspected genital herpes who were treated in Linyi Dermatology Hospital from January 2017 to October 2022 were randomly selected,and their specimens were collected and detected by ELISA and PCR respectively.With the results of virus culture as the gold standard,the application of ELISA and PCR in the detection of genital herpes virus antigen was analyzed.Results:There was no significant difference in the detection rate of HSV-1 infection,HSV-2 infection,mixed infection and total positive detection rate between the two detection methods(P>0.05).With the results of virus culture as the gold standard,there were no significant differences in sensitivity,accuracy,specificity,positive predictive value and negative predictive value between ELISA and PCR in the diagnosis of genital herpes(P>0.05).Conclusion:Both ELISA and PCR are of high application value in the detection of genital herpes virus antigen,but ELISA is economical,practical and easy to operate,which is more suitable for promotion and application i n primary hospitals.
作者
孙伟
王炜
SUN Wei;WANG Wei(Linyi Dermatology Hospital,Linyi 276004,Shandong,China)
关键词
生殖器疱疹病毒
抗原
酶联免疫吸附法
聚合酶链反应法
检测效果
genital herpes virus
Antigen
Enzyme-linked immunosorbent ass ay
Polymerase chain reaction method
Detection effect
