摘要
目的比较志不安于肾和魄不安于肺不寐大鼠血清和脑γ-氨基丁酸(GABA)及谷氨酸(Glu)水平的差异,为中医不寐五神分型诊断法鉴别魄不安于肺和志不安于肾不寐提供依据。方法将36只雌性SD大鼠随机分为志不安于肾组(肾组)、魄不安于肺组(肺组)和正常组。采用久坐湿地法制备志不安于肾不寐大鼠模型,采用狭长平台浅水环境法制备魄不安于肺不寐大鼠模型。以睡眠监测评价睡眠剥夺情况,以水迷宫实验评价学习记忆功能障碍,以酶联免疫吸附试验(ELISA)评价血清GABA、Glu及脑Glu水平,以免疫组化法评价不同脑区的GABA水平。结果与正常组比较,肾组和肺组睡眠时间均缩短,肾组觉醒次数减少,肺组觉醒次数增加。肾组表现为睡眠后期不能再次入睡致睡眠时间减少,肺组表现为上午和下午频繁觉醒致睡眠时间减少(P<0.05)。肾组和肺组逃避潜伏期均延长,穿越平台次数均减少(P<0.05)。肾组逃避潜伏期长于肺组,穿越平台次数少于肺组(P<0.05)。肺组血清GABA和Glu水平显著降低,肾组血清和脑Glu水平显著降低,且显著低于肺组(P<0.05);肾组和肺组下丘脑、丘脑、压部b区、运动区等区域GABA水平均显著降低,肺组显著低于肾组(P<0.05);听觉区和海马杏仁移行区肺组GABA水平低于肾组,肾组海马杏仁移行区GABA水平高于正常组(P<0.05)。结论志不安于肾和魄不安于肺不寐大鼠均存在着学习记忆功能障碍,但志不安于肾不寐大鼠较为严重;志不安于肾不寐大鼠以Glu水平下降为主,魄不安于肺不寐大鼠以GABA水平下降为主,说明中医不寐五神分型诊断法中志不安于肾和魄不安于肺不寐之间Glu、GABA等分子水平存在差异。
Objective To compare serum and brain Gamma-aminobutyric acid(GABA)as well as glutamic acid(Glu)in the sleep deprived rats due to Zhi unrest in the kidney or Po unrest in the lung,in order to provide reference for their classification and diagnosis of insomnia in traditional Chinese medicine based on five-spirit.Methods Thirty-six female Sprague Dawley(SD)rats were randomly divided into three groups:Zhi unrest in the kidney group(kidney group),Po unrest in the lung group(lung group)and normal group.The sleep deprived model of Zhi unrest in the kidney was prepared by long time sitting on the cold and wet sand in the rat cage.The model of Po unrest in the lung was prepared by the shallow water environment in narrow and long platforms.The sleep was evaluated through sleep monitor and the learning and memory function was evaluated by Water Morris Maze test.The levels of serum GABA and Glu as well as brain Glu were detected by enzyme-linked immunosorbent assay(ELISA),and the levels of GABA in different brain regions were detected by immunohistochemistry.Results Compared with the normal group,the sleep time was shortened in the 2 model groups.The arousal times increased in the lung group and decreased in the kidney group.The kidney group showed a decrease in sleep time due to inability to fall asleep again in the late stage of sleep,while the lung group showed a decrease in sleep time caused by frequent awakening in the morning and afternoon(P<0.05).The escape latency in the kidney group was longer and the number of times crossing the platform in the kidney group was less than those in the lung group(P<0.05).The levels of serum GABA and Glu decreased significantly in the lung group.The levels of serum and brain Glu decreased significantly in the kidney group and lower than that in the lung group.The levels of GABA in hypothalamus,thalamus,retrosplenial granular b cortex,motor cortex,etc.,decreased significantly in the two model groups with lower levels in the lung group(P<0.05).The level of GABA in auditory area and hip
作者
闫德祺
张星平
么嵌巍
张文慧
和玉松
崔瑞
佟振鹏
王凯凯
梁瑞宁
陈旭
YAN Deqi;ZHANG Xingping;YAO Qianwei;ZHANG Wenhui;HE Yusong;CUI Rui;TONG Zhenpeng;WANGKaikai;LIANG Ruining;CHEN Xu(Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830017,China)
出处
《上海中医药杂志》
CSCD
2023年第3期87-92,97,共7页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(82160873、81960837、81560762、81260526)
新疆维吾尔自治区中医学重点学科项目(1005)
新疆医科大学研究生创新创业项目(CXCY2021023)。
关键词
失眠
五脏藏神
Γ-氨基丁酸
谷氨酸
大鼠模型
中药研究
insomnia
Five Zangs Storing Spirits
GABA
Glu
rat model
traditional Chinese herbal medicine research
